PMID- 12767483
OWN - NLM
STAT- MEDLINE
DCOM- 20030725
LR  - 20220331
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 46
IP  - 2
DP  - 2003 Jun
TI  - Unaltered pain-related behavior in mice lacking NMDA receptor GluRepsilon 1 
      subunit.
PG  - 199-204
AB  - Noxious afferent input following tissue damage and inflammation triggers a state 
      of neuronal hyperexcitability-a phenomenon of central sensitization-which 
      manifests behaviorally as allodynia and hyperalgesia. At the molecular level, 
      maintenance of central sensitization is largely dependent on the 
      N-methyl-D-aspartate receptor (NMDAR) activation. NMDARs are composed of 
      GluRzeta1 (NR1) and one of four GluRepsilon (NR2) subunits, which determine the 
      functional properties of native NMDARs. Although there is accumulating evidence 
      to implicate GluRepsilon 2-containing NMDARs in pain mechanisms, the functional 
      significance of GluRepsilon 1-containing NMDARs in this setting has not been 
      examined in detail. Here, we used hind paw injection of formalin, complete 
      Freund's adjuvant and a nerve injury model to investigate the effects of 
      GluRepsilon 1 subunit gene deletion on pain-related behavior in mice. In all of 
      the models tested, GluRepsilon 1-deficient mice exhibited responses similar to 
      wild-type controls. These results suggest that GluRepsilon 1 disruption does not 
      result in altered nociceptive behavior in mice. Although the contribution of 
      other nociceptive pathways cannot be ruled out, we speculate that the preserved 
      function of GluRepsilon 2-containing NMDARs could explain unaltered nociceptive 
      behavior in mutant mice.
FAU - Petrenko, Andrei B
AU  - Petrenko AB
AD  - Department of Cellular Neurobiology, Brain Research Institute, Niigata 
      University, 1 Asahimachi-dori, Japan.
FAU - Yamakura, Tomohiro
AU  - Yamakura T
FAU - Baba, Hiroshi
AU  - Baba H
FAU - Sakimura, Kenji
AU  - Sakimura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Irritants)
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Behavior, Animal/*physiology
MH  - Blotting, Western
MH  - Formaldehyde/pharmacology
MH  - Freund's Adjuvant/pharmacology
MH  - Hindlimb/drug effects/innervation/physiology
MH  - Irritants/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Nociceptors/metabolism
MH  - Pain/*genetics/*metabolism
MH  - Pain Measurement
MH  - Pain Threshold/physiology
MH  - Physical Stimulation
MH  - Reaction Time/genetics
MH  - Receptors, N-Methyl-D-Aspartate/*deficiency/genetics/metabolism
EDAT- 2003/05/28 05:00
MHDA- 2003/07/26 05:00
CRDT- 2003/05/28 05:00
PHST- 2003/05/28 05:00 [pubmed]
PHST- 2003/07/26 05:00 [medline]
PHST- 2003/05/28 05:00 [entrez]
AID - S0168010203000610 [pii]
AID - 10.1016/s0168-0102(03)00061-0 [doi]
PST - ppublish
SO  - Neurosci Res. 2003 Jun;46(2):199-204. doi: 10.1016/s0168-0102(03)00061-0.