PMID- 12768369
OWN - NLM
STAT- MEDLINE
DCOM- 20030930
LR  - 20061115
IS  - 0941-1291 (Print)
IS  - 0941-1291 (Linking)
VI  - 33
IP  - 6
DP  - 2003
TI  - Identification of differentially expressed mRNAs of the autologous vein graft 
      after an arterial bypass in mongrel dogs.
PG  - 434-40
AB  - PURPOSE: Intimal hyperplasia (IH) is characterized by vascular smooth muscle cell 
      (VSMC) proliferation in the intima and subsequent accumulation of extracellular 
      matrix. A variety of factors that might be considered as possible VSMC mitogens 
      induce the specific gene expression of VSMC. This study was designed to identify 
      differentially expressed mRNA by method using an mRNA differential display. 
      METHODS: A bilateral femoropopliteal reverse saphenous vein bypass was performed 
      on both hind limbs of mongrel dogs. At 4 and 8 weeks after the implantation, the 
      vein bypass grafts were harvested. The total RNAs were extracted from each 
      specimen and transcribed into the cDNAs. Amplified cDNAs using 16 sets of primers 
      were separated on DNA sequencing gel. Differentially displayed bands were excised 
      from the gel, cloned, and then sequenced. The sequences were compared with the 
      National Center of Biotechnology Information nonredundant sequence database using 
      the Basic Local Alignment Search Tool (BLAST) program. RESULTS: Forty-three bands 
      were cloned and sequenced. A computer search revealed that 11 of them had 
      similarities to known genes while 32 cDNAs had no similarities to any registered 
      DNA sequence. CONCLUSIONS: We found several genes to be related to IH. Some of 
      them had already had their function and sequence identified whereas some of them 
      have yet to be identified. Further studies are necessary to determine the precise 
      relationship between these genes and IH.
FAU - Kim, Dong-Ik
AU  - Kim DI
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, 50 Ilwon-Dong, Kangnam-Ku, Seoul 135-710, South Korea.
FAU - Kim, Ji-Eun
AU  - Kim JE
FAU - Huh, Se-Ho
AU  - Huh SH
FAU - Park, Jung-Hwan
AU  - Park JH
FAU - Lee, Byung-Boong
AU  - Lee BB
FAU - Han, Jin-Soo
AU  - Han JS
FAU - Kim, Jong-Sung
AU  - Kim JS
FAU - Lee, Min-Jae
AU  - Lee MJ
FAU - Jang, In-Sung
AU  - Jang IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Surg Today
JT  - Surgery today
JID - 9204360
RN  - 0 (Mitogens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Anastomosis, Surgical
MH  - Animals
MH  - Arteries/surgery
MH  - Dogs
MH  - *Gene Expression
MH  - *Gene Expression Profiling
MH  - Hyperplasia
MH  - Male
MH  - Mitogens/pharmacology
MH  - Muscle, Smooth, Vascular/cytology/metabolism
MH  - RNA, Messenger/genetics
MH  - Saphenous Vein/transplantation
MH  - Tunica Intima/*pathology
MH  - Veins/pathology/*transplantation
EDAT- 2003/05/28 05:00
MHDA- 2003/10/01 05:00
CRDT- 2003/05/28 05:00
PHST- 2003/05/28 05:00 [pubmed]
PHST- 2003/10/01 05:00 [medline]
PHST- 2003/05/28 05:00 [entrez]
AID - 10.1007/s10595-002-2516-x [doi]
PST - ppublish
SO  - Surg Today. 2003;33(6):434-40. doi: 10.1007/s10595-002-2516-x.