PMID- 12768577
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20151119
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 49
IP  - 6
DP  - 2003 Jun
TI  - Receptor-mediated endocytosis of iron-oxide particles provides efficient labeling 
      of dendritic cells for in vivo MR imaging.
PG  - 1006-13
AB  - Dendritic cells (DCs) function as antigen presenting cells in vivo and play a 
      fundamental role in numerous diseases. New methods are described for 
      high-efficiency intracellular labeling of DCs with superparamagnetic iron-oxide 
      (SPIO) utilizing a receptor-mediated endocytosis (RME) mechanism. Bone 
      marrow-derived DCs or a fetal skin-derived DC line were incubated with SPIO 
      conjugated to anti-CD11c monoclonal antibody (mAb) under conditions favoring RME. 
      These cells exhibited approximately a 50-fold increase in uptake relative to DCs 
      incubated with SPIO without the mAb. Flow cytometry studies assaying cell surface 
      markers showed a down-modulation of CD11c, but no other changes in phenotype. 
      Immunological function of the DCs was unmodified by the labeling, as determined 
      by cytokine secretion assays. The RME mechanism was confirmed using electron 
      microscopy, endocytosis inhibition assays, and incubation experiments with SPIO 
      conjugated to mAbs against accessory molecules that are not expressed on DCs. 
      Labeled DCs were injected into murine quadriceps and monitored in vivo for 
      several days using MR microimaging at 11.7 T. DCs were observed to remain within 
      the muscle for >24 hr. The use of RME is an efficient way to label immune cells 
      for in vivo MRI and can be applied to a wide variety of cell types.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Ahrens, E T
AU  - Ahrens ET
AD  - Department of Biological Sciences and the Pittsburgh NMR Center for Biomedical 
      Research, Carnegie Mellon University, Pennsylvania 15213, USA. eta@andrew.cmu.edu
FAU - Feili-Hariri, M
AU  - Feili-Hariri M
FAU - Xu, H
AU  - Xu H
FAU - Genove, G
AU  - Genove G
FAU - Morel, P A
AU  - Morel PA
LA  - eng
GR  - CA73743/CA/NCI NIH HHS/United States
GR  - P41-RR03631/RR/NCRR NIH HHS/United States
GR  - P50-AR049617/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Ferric Compounds)
RN  - 0 (Receptors, Cell Surface)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Dendritic Cells/immunology/*physiology
MH  - Endocytosis/*physiology
MH  - *Ferric Compounds
MH  - Magnetic Resonance Spectroscopy
MH  - Mice
MH  - Microscopy, Electron
MH  - Phenotype
MH  - Receptors, Cell Surface/*metabolism
EDAT- 2003/05/28 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/05/28 05:00
PHST- 2003/05/28 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/05/28 05:00 [entrez]
AID - 10.1002/mrm.10465 [doi]
PST - ppublish
SO  - Magn Reson Med. 2003 Jun;49(6):1006-13. doi: 10.1002/mrm.10465.