PMID- 12768634
OWN - NLM
STAT- MEDLINE
DCOM- 20030826
LR  - 20191210
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 28
IP  - 6
DP  - 2003 Jun
TI  - Evaluation of fluorescence in situ hybridization as an ancillary tool to urine 
      cytology in diagnosing urothelial carcinoma.
PG  - 301-7
AB  - Our purpose was to evaluate the feasibility of performing fluorescence in situ 
      hybridization (FISH) on routine urine samples and to compare the relative 
      sensitivities of urine cytology and FISH for detecting urothelial carcinoma. 
      Light microscopy (LM) using cytologic evaluation and FISH were used to study 121 
      consecutive urine samples. A mixture of fluorescent probes to chromosomes 3, 7, 
      17, and the 9p21 locus were used for detection of numerical chromosomal 
      abnormalities (UroVysion, Vysis/Abbott). Biopsy specimens from patients in the 
      study were reviewed if available. FISH analysis was performed without knowledge 
      of cytology or biopsy findings. The urine cytology of 121 samples was interpreted 
      as 59 negative, 41 reactive, 16 atypical, 2 suspicious and 3 insufficient cells 
      for diagnosis. 85 samples were successfully analyzed by FISH. Thirty-one of these 
      showed chromosomal abnormalities and these samples were initially regarded on the 
      original cytology reading as follows: 10 negative, 10 reactive, 9 atypical, and 2 
      suspicious. FISH demonstrated chromosomal abnormalities in a significant number 
      of cases (67%) that were initially diagnosed as normal or reactive by LM. 
      Twenty-five patients were identified who had biopsy-proven TCC and successful 
      FISH. Thirteen of the 25 patients (52%) were abnormal by FISH (cytology: 2 
      suspicious, 6 atypical, 4 reactive, 1 negative). One patient was atypical by 
      cytology with normal FISH results but had TCC on biopsy. Hyperdiploidy for 
      chromosomes 3 (77%) and 7 (67%) were seen consistently. Multiple chromosomal 
      abnormalities were seen in 67% of these cases. We conclude that FISH has a 
      greater sensitivity in detecting urothelial carcinoma when coupled with urine 
      cytology. It is not entirely clear at this time whether a positive FISH may 
      indicate frank neoplastic urothelial transformation or merely be an indicator of 
      unstable urothelium capable of or primed for malignant transformation thus 
      detecting patients at significant risk. The use of FISH in conjunction with urine 
      cytology can potentially reduce urothelial carcinoma morbidity and mortality by 
      diagnosing these tumors earlier.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Veeramachaneni, Ravindra
AU  - Veeramachaneni R
AD  - Department of Pathology, Louisiana State University Health Sciences Center, 
      Shreveport, Louisiana 71130, USA.
FAU - Nordberg, Mary L
AU  - Nordberg ML
FAU - Shi, Runhua
AU  - Shi R
FAU - Herrera, Guillermo A
AU  - Herrera GA
FAU - Turbat-Herrera, Elba A
AU  - Turbat-Herrera EA
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Carcinoma, Transitional Cell/genetics/*pathology/urine
MH  - Chromosome Aberrations
MH  - Cytodiagnosis/*methods
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/genetics/*pathology/urine
MH  - Urine/*cytology
EDAT- 2003/05/28 05:00
MHDA- 2003/08/27 05:00
CRDT- 2003/05/28 05:00
PHST- 2003/05/28 05:00 [pubmed]
PHST- 2003/08/27 05:00 [medline]
PHST- 2003/05/28 05:00 [entrez]
AID - 10.1002/dc.10291 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2003 Jun;28(6):301-7. doi: 10.1002/dc.10291.