PMID- 12768709
OWN - NLM
STAT- MEDLINE
DCOM- 20040330
LR  - 20061115
IS  - 0412-3948 (Print)
IS  - 0412-3948 (Linking)
VI  - 35
IP  - 4
DP  - 2000 Aug
TI  - [The study of p16 gene mutation and methylation in laryngeal squamous cell 
      carcinoma].
PG  - 292-4
AB  - OBJECTIVE: To investigate the development and mechanism of the p16 gene 
      inactivation in laryngeal squamous cell carcinoma (LSCC). METHODS: With PCR-based 
      methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and 
      methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue 
      were studied. RESULTS: No mutation were found in first and second exon in the 
      samples; methylation of Cfo I were detected in 6 cases in first exon, methylation 
      of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second 
      exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 
      cases. CONCLUSION: Methylation of promoter in p16 gene is one of the important 
      mechanisms of the inactivation of the gene. The inactivation of p16 gene has a 
      close relation with the development and progress of LSCC.
FAU - Bai, S
AU  - Bai S
AD  - Department of Otorhinolaryngology, Second Clinical College, Chinese Medical 
      University, Shenyang 110003, China. baisu@pub.sy.ln.cn
FAU - Gao, H
AU  - Gao H
FAU - Liang, H
AU  - Liang H
FAU - Fei, S
AU  - Fei S
FAU - Zhang, X
AU  - Zhang X
FAU - Sun, K
AU  - Sun K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Ke Za Zhi
JT  - Zhonghua er bi yan hou ke za zhi
JID - 16210350R
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *DNA Methylation
MH  - Exons
MH  - *Genes, p16
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Mutation
EDAT- 2003/05/29 05:00
MHDA- 2004/03/31 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2004/03/31 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000 Aug;35(4):292-4.