PMID- 12768773
OWN - NLM
STAT- MEDLINE
DCOM- 20040330
LR  - 20061115
IS  - 0412-3948 (Print)
IS  - 0412-3948 (Linking)
VI  - 35
IP  - 3
DP  - 2000 Jun
TI  - [Detection of bcl-2/JH gene rearrangement by semi-nested polymerase chain 
      reaction from fresh tumor samples in patients with laryngeal squamous cell 
      carcinomas].
PG  - 185-7
AB  - OBJECTIVE: To evaluate the clinical implications of bcl-2/JH gene rearrangement 
      in laryngeal squamous cell carcinomas (LSCC). METHODS: Bcl-2/JH gene 
      rearrangement analysis in fresh tumor samples was performed in 60 patients with 
      LSCC by semi-nested polymerase chain reaction(PCR). RESULTS: The results showed 
      that bcl-2/JH gene rearrangement was found in 37 out of 60 patients. The 
      breakpoint was located within the major breakpoint region(mbr) in 33 of the 60 
      patients and the remaining patients had bcl-2 translocation within the minor 
      cluster region(mcr). The results of the study showed that the rearrangement rate 
      of bcl-2 gene was not related to the grade of differentiation, clinical stage, 
      and neck lymph-node metastasis (P > 0.05), and it was related to heavy smoking (P 
      < 0.05). CONCLUSION: Detection of bcl-2/JH gene rearrangement could reveal that 
      bcl-2/JH fusion gene in LSCC is an important molecular biological marker and has 
      a significant role in occurrence and development of LSCC.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Otorhinolaryngology, Forth Affiliated Hospital, West China 
      University of Medical Sciences, Chengdu 610041, China. 200299@mail.guoli.com.cn
FAU - Liu, S
AU  - Liu S
FAU - Tang, S
AU  - Tang S
FAU - Lin, D
AU  - Lin D
FAU - Liang, C
AU  - Liang C
FAU - An, H
AU  - An H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Ke Za Zhi
JT  - Zhonghua er bi yan hou ke za zhi
JID - 16210350R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Female
MH  - Gene Rearrangement
MH  - Genes, bcl-2/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
EDAT- 2003/05/29 05:00
MHDA- 2004/03/31 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2004/03/31 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000 Jun;35(3):185-7.