PMID- 12770552
OWN - NLM
STAT- MEDLINE
DCOM- 20030730
LR  - 20220316
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 119
IP  - 2
DP  - 2003
TI  - A saturated-fat diet aggravates the outcome of traumatic brain injury on 
      hippocampal plasticity and cognitive function by reducing brain-derived 
      neurotrophic factor.
PG  - 365-75
AB  - We have conducted studies to determine the potential of dietary factors to affect 
      the capacity of the brain to compensate for insult. Rats were fed with a high-fat 
      sucrose (HFS) diet, a popularly consumed diet in industrialized western 
      societies, for 4 weeks before a mild fluid percussion injury (FPI) or sham 
      surgery was performed. FPI impaired spatial learning capacity in the Morris water 
      maze, and these effects were aggravated by previous exposure of the rats to the 
      action of the HFS diet. Learning performance decreased according to levels of 
      brain-derived neurotrophic factor (BDNF) in individual rats, such that rats with 
      the worst learning efficacy showed the lowest levels of BDNF in the hippocampus. 
      BDNF immunohistochemistry localized the decreases in BDNF to the CA3 and dentate 
      gyrus of the hippocampal formation. BDNF has a strong effect on synaptic 
      plasticity via the action of synapsin I and cAMP-response element-binding protein 
      (CREB), therefore, we assessed changes in synapsin I and CREB in conjunction with 
      BDNF. Levels of synapsin I and CREB decreased in relation to decreases in BDNF 
      levels. The combination of FPI and the HFS diet had more dramatic effects on the 
      active state (phosphorylated) of synapsin I and CREB. There were no signs of 
      neurodegeneration in the hippocampus of any rat group assessed with Fluoro-Jade B 
      staining. The results suggest that FPI and diet impose a risk factor to the 
      molecular machinery in charge of maintaining neuronal function under homeostatic 
      and challenging situations.
FAU - Wu, A
AU  - Wu A
AD  - Department of Physiological Science, University of California at Los Angeles, 621 
      Charles E Young Drive, Los Angeles, CA H-1450, USA.
FAU - Molteni, R
AU  - Molteni R
FAU - Ying, Z
AU  - Ying Z
FAU - Gomez-Pinilla, F
AU  - Gomez-Pinilla F
LA  - eng
GR  - 38978/PHS HHS/United States
GR  - 39522/PHS HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Dietary Fats)
RN  - 0 (Dietary Sucrose)
RN  - 0 (Fatty Acids)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Synapsins)
RN  - 0 (fluoro jade)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Blotting, Western
MH  - Brain Injuries/*metabolism/pathology/physiopathology
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cognition/*drug effects
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Dietary Fats/*adverse effects
MH  - Dietary Sucrose/adverse effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fatty Acids/*adverse effects
MH  - Fluoresceins
MH  - Fluorescent Dyes/pharmacokinetics
MH  - Hippocampus/*drug effects/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Maze Learning/*drug effects
MH  - Neuronal Plasticity/*drug effects
MH  - Organic Chemicals
MH  - Percussion/methods
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reaction Time/drug effects
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synapsins/biosynthesis/metabolism
MH  - Time Factors
EDAT- 2003/05/29 05:00
MHDA- 2003/07/31 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2003/07/31 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
AID - S0306452203001544 [pii]
AID - 10.1016/s0306-4522(03)00154-4 [doi]
PST - ppublish
SO  - Neuroscience. 2003;119(2):365-75. doi: 10.1016/s0306-4522(03)00154-4.