PMID- 12770789
OWN - NLM
STAT- MEDLINE
DCOM- 20040211
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 6
DP  - 2003 Jun
TI  - The detection and definition of IgM alloantibodies in the presence of IgM 
      autoantibodies using flowPRA beads.
PG  - 593-9
AB  - We have developed a flow cytometry-based screening method using FlowPRA (One 
      Lambda) human leukocyte antigen (HLA) class I panel beads and FlowPRA (One 
      Lambda) HLA class I specificity beads for the detection and definition of 
      immunoglobulin (Ig)M HLA-specific antibodies in the presence of IgM 
      autoantibodies. Forty-six autoantibody-positive patients who were on the waiting 
      list for a renal transplant (56 sera) were tested in parallel with FlowPRA (One 
      Lambda) HLA class I beads and FlowPRA (One Lambda) control beads. Sera that were 
      positive for IgM HLA class I antibodies were subsequently tested with FlowPRA HLA 
      class I specificity beads to determine the HLA specificities. Thirteen of the 46 
      patients were positive for IgM HLA class I-specific antibodies. Eleven of the 13 
      had previous failed transplants and 2 were awaiting a primary transplant. For 9 
      of the 13 positive patients, IgM HLA class I specificities were defined. We have 
      demonstrated the presence of IgM HLA-specific antibodies in patients with IgM 
      autoantibodies. This study demonstrates the value of FlowPRA HLA class I panel 
      and specificity beads for the detection and definition of IgM HLA class 
      I-specific antibodies.
FAU - Khan, Naheed
AU  - Khan N
AD  - Transplantation Laboratory, Manchester Royal Infirmary, Manchester, United 
      Kingdom. Naheed.Khan@cmmc.nhs.uk
FAU - Robson, Amanda J
AU  - Robson AJ
FAU - Worthington, Judith E
AU  - Worthington JE
FAU - Martin, Susan
AU  - Martin S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Autoantibodies/*immunology
MH  - Humans
MH  - Immunoglobulin M/*immunology
MH  - Isoantibodies/*analysis/immunology
MH  - Kidney Transplantation/immunology
MH  - Microspheres
EDAT- 2003/05/29 05:00
MHDA- 2004/02/12 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2004/02/12 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
AID - S019888590300065X [pii]
AID - 10.1016/s0198-8859(03)00065-x [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Jun;64(6):593-9. doi: 10.1016/s0198-8859(03)00065-x.