PMID- 12771236
OWN - NLM
STAT- MEDLINE
DCOM- 20040503
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 9
IP  - 6
DP  - 2003 Jun
TI  - Gestational profile of matrix metalloproteinases in rat uterine artery.
PG  - 351-8
AB  - Mechanisms underlying structural reorganization of the uterine artery in 
      pregnancy remain largely unknown. Matrix metalloproteinases (MMPs) which are 
      involved in degradation of vascular wall matrix are likely to play a key role. In 
      this investigation of rat uterine artery, key MMPs and the specific tissue 
      inhibitors of MMPs (TIMPs) together with three housekeeping genes were studied 
      before, during and after pregnancy, using real time PCR. Data were analysed by 
      partial least squares analysis as well as by conventional univariate methods. 
      Each gene studied [MMP-2, MMP-3, MMP-7, MMP-9, MMP-12, MMP-13, membrane-type 1 
      (MT1)-MMP, TIMP-1, TIMP-2, GAPDH, cyclophilin and beta-actin] increased in late 
      pregnancy (day 21). MMP-2, MT1MMP, MMP-3 and TIMP-1 transcripts were also 
      elevated at day 7. TIMP-1 and MMP-3 mRNA expression returned to virgin control 
      values in the post-partum, whereas others remained elevated or increased further 
      (MMP-9, MMP-13). Gelatin zymography showed maximum elevation of MMP-2 at day 21. 
      A novel 43-45 kDa gelatinolytic doublet was observed which increased in density 
      with gestation and may represent an active MMP-2 fragment. Together, these data 
      strongly suggest that MMPs and TIMPs are likely to play an important role in 
      remodelling uterine arteries in rat pregnancy and may represent means by which 
      vasodilatation is maintained in later pregnancy. Continued elevated levels of 
      some MMPs post-partum may contribute to vessel regression and return to a 
      non-pregnant physiological state.
FAU - Kelly, B A
AU  - Kelly BA
AD  - Maternal and Fetal Research Unit, Department of Womens Health, Guy's, King's and 
      St Thomas' School of Medicine, 10th Floor St Thomas' Hospital, Lambeth Palace 
      Road, London SE1 7EH, UK. brenda.a.kelly@kcl.ac.uk
FAU - Bond, B C
AU  - Bond BC
FAU - Poston, L
AU  - Poston L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Arteries/*enzymology
MH  - Female
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Tissue Inhibitor of Metalloproteinases/genetics/metabolism
MH  - Uterus/*blood supply
EDAT- 2003/05/29 05:00
MHDA- 2004/05/05 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2004/05/05 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
AID - 10.1093/molehr/gag043 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2003 Jun;9(6):351-8. doi: 10.1093/molehr/gag043.