PMID- 12771423
OWN - NLM
STAT- MEDLINE
DCOM- 20030630
LR  - 20200226
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 84
IP  - Pt 6
DP  - 2003 Jun
TI  - The major envelope protein, GP5, of a European porcine reproductive and 
      respiratory syndrome virus contains a neutralization epitope in its N-terminal 
      ectodomain.
PG  - 1535-1543
LID - 10.1099/vir.0.18957-0 [doi]
AB  - A set of neutralizing monoclonal antibodies (mAbs) directed against the GP(5) 
      protein of European type porcine reproductive and respiratory syndrome virus 
      (PRRSV) has been produced previously (Weiland et al., 1999). This set reacted 
      with a plaque-purified virus (PPV) subpopulation of Dutch isolate Intervet-10 
      (I-10), but not with the European prototype PRRSV LV. In order to map the 
      neutralization epitope in the GP(5) protein of the PPV strain, the ORF5 
      nucleotide sequence of PPV was determined. When the amino acid sequence derived 
      from this nucleotide sequence was compared with that of PRRSV LV, four amino acid 
      differences were found. Using site-directed mutagenesis, we showed that a proline 
      residue at position 24 of the GP(5) sequence of the PPV strain enabled 
      recognition by the neutralizing mAbs. Pepscan analysis demonstrated that the 
      epitope recognized by the neutralizing mAbs stretched from residues 29 to 35. 
      Surprisingly, the reactivity of the mAbs in the Pepscan system was independent of 
      the presence of a proline in position 24. Moreover, residue 24 is located within 
      the predicted signal peptide, implying that either the signal peptide is not 
      cleaved or is cleaved due to the presence of Pro(24) such that the epitope 
      remains intact. Our results demonstrate the presence of a neutralization epitope 
      in the N-terminal ectodomain of the GP(5) protein of PRRSV and imply a role for 
      the ectodomain of GP(5) in the infection of PRRSV.
FAU - Wissink, E H J
AU  - Wissink EHJ
AD  - Institute for Animal Science and Health (ID-Lelystad), Division of Infectious 
      Diseases and Food Chain Quality, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - van Wijk, H A R
AU  - van Wijk HAR
AD  - Institute for Animal Science and Health (ID-Lelystad), Division of Infectious 
      Diseases and Food Chain Quality, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Kroese, M V
AU  - Kroese MV
AD  - Institute for Animal Science and Health (ID-Lelystad), Division of Infectious 
      Diseases and Food Chain Quality, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Weiland, E
AU  - Weiland E
AD  - Federal Research Centre for Viruses Diseases of Animals, Tubingen, Germany.
FAU - Meulenberg, J J M
AU  - Meulenberg JJM
AD  - Institute for Animal Science and Health (ID-Lelystad), Division of Infectious 
      Diseases and Food Chain Quality, PO Box 65, 8200 AB Lelystad, The Netherlands.
FAU - Rottier, P J M
AU  - Rottier PJM
AD  - Virology Division, Utrecht University, Utrecht, The Netherlands.
FAU - van Rijn, P A
AU  - van Rijn PA
AD  - Institute for Animal Science and Health (ID-Lelystad), Division of Infectious 
      Diseases and Food Chain Quality, PO Box 65, 8200 AB Lelystad, The Netherlands.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibodies, Viral
MH  - Antigens, Viral/chemistry/genetics
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Line
MH  - Cricetinae
MH  - DNA, Viral/genetics
MH  - Epitope Mapping
MH  - Epitopes/chemistry/genetics
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neutralization Tests
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Proline/genetics
MH  - Recombinant Proteins/chemistry/genetics/immunology
MH  - Sus scrofa
MH  - Transfection
MH  - Viral Envelope Proteins/chemistry/genetics/*immunology
MH  - Viral Proteins/genetics/immunology
EDAT- 2003/05/29 05:00
MHDA- 2003/07/02 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
AID - 10.1099/vir.0.18957-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2003 Jun;84(Pt 6):1535-1543. doi: 10.1099/vir.0.18957-0.