PMID- 12771503
OWN - NLM
STAT- MEDLINE
DCOM- 20031117
LR  - 20171101
IS  - 0250-8095 (Print)
IS  - 0250-8095 (Linking)
VI  - 23
IP  - 4
DP  - 2003 Jul-Aug
TI  - Direct contact between human peripheral blood mononuclear cells and renal 
      fibroblasts facilitates the expression of monocyte chemoattractant protein-1.
PG  - 208-13
AB  - BACKGROUND: Cell-to-cell interaction is thought to be an important feature of a 
      variety of biological processes. As far as the proinflammatory process is 
      concerned, the interaction between mesangial cells and monocytes/macrophages 
      induces the expression of monocyte chemoattractant protein-1 (MCP-1), and this 
      may play a role in glomerulonephritis. In this study, we investigated whether the 
      cell-to-cell interaction between immune cells and renal fibroblasts induces MCP-1 
      gene expression, which may be involved in interstitial inflammation in the 
      kidney. METHODS: Human renal fibroblast cell lines, tNKF (from a normal kidney) 
      and tFKIF (from a kidney with fibrosis), and peripheral blood mononuclear cells 
      (PBMC) were used to assess the effect of cell-to-cell contact on the expression 
      of MCP-1 mRNA in the fibroblasts. The expression of the MCP-1 gene in the 
      fibroblasts was also examined after stimulation with tumor necrosis factor-alpha 
      (TNF-alpha) and the culture supernatant from PBMC. RT-PCR was used to detect 
      MCP-1 mRNA expression. Neutralizing antibodies to intercellular adhesion 
      molecule-1 (ICAM-1) and vascular endothelial adhesion molecule-1 (VCAM-1) were 
      used to block the cell-to-cell contact between the fibroblasts and PBMC. RESULTS: 
      TNF-alpha and the culture supernatant from PBMC increased MCP-1 gene expression 
      in tNKF cells. Contact culture with PBMC also significantly increased MCP-1 gene 
      expression in tNKF cells. Although the basal level of MCP-1 mRNA was higher in 
      tFKIF than tNKF cells, tFKIF cells did not respond significantly to any 
      stimulation in this study. Following pretreatment with anti-ICAM-1 antibody, 
      MCP-1 gene expression in tNKF cells was significantly suppressed in contact 
      culture with PBMC. Anti-VCAM-1 antibody treatment had no effects. CONCLUSION: It 
      is suggested that the interaction between renal fibroblasts and PBMC was mediated 
      through direct contact and by secreted humoral factors. ICAM-1 on renal 
      fibroblasts may be involved in the direct cell-to-cell interaction inducing MCP-1 
      gene expression, which seems to be involved in renal interstitial inflammation.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Hao, Lirong
AU  - Hao L
AD  - Department of Nephrology, Saitama Medical School, Irumagun, Saitama, Japan.
FAU - Okada, Hirokazu
AU  - Okada H
FAU - Kanno, Yoshihiko
AU  - Kanno Y
FAU - Inoue, Tsutomu
AU  - Inoue T
FAU - Kobayashi, Tatsuya
AU  - Kobayashi T
FAU - Watanabe, Yusuke
AU  - Watanabe Y
FAU - Strutz, Frank
AU  - Strutz F
FAU - Muller, Gerhard A
AU  - Muller GA
FAU - Suzuki, Hiromichi
AU  - Suzuki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030527
PL  - Switzerland
TA  - Am J Nephrol
JT  - American journal of nephrology
JID - 8109361
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Antibodies/pharmacology
MH  - *Cell Communication
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Coculture Techniques
MH  - Fibroblasts/cytology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/immunology
MH  - Kidney/*cytology
MH  - Leukocytes, Mononuclear/*cytology
MH  - RNA, Messenger/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/immunology
EDAT- 2003/05/29 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/05/29 05:00
PHST- 2003/03/20 00:00 [received]
PHST- 2003/04/23 00:00 [accepted]
PHST- 2003/05/29 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/05/29 05:00 [entrez]
AID - 71480 [pii]
AID - 10.1159/000071480 [doi]
PST - ppublish
SO  - Am J Nephrol. 2003 Jul-Aug;23(4):208-13. doi: 10.1159/000071480. Epub 2003 May 
      27.