PMID- 12773243
OWN - NLM
STAT- MEDLINE
DCOM- 20050310
LR  - 20191224
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 11
IP  - 5
DP  - 2003 May
TI  - [Effect of HanDanGanLe on the cytokines in fibrotic rats].
PG  - 285-7
AB  - OBJECTIVE: To study the expression of connective tissue growth factor (CTGF) mRNA 
      and transforming growth factor beta 1 (TGFbeta1) mRNA in immunity-induced liver 
      fibrosis rats and the effect of HanDanGanLe on them. METHODS: Male wistar rats 
      were given intraperitoneal injection of porcine serum twice a week. At the 
      beginning, the rats in HanDanGanLe-treatment group were feed with HanDanGanLe for 
      precaution. The rats were killed after twelve weeks, then CTGF mRNA and TGFbeta1 
      mRNA were detected in liver samples with in situ hybridization, and the formation 
      of liver fibrosis was observed with HE stain. The semi-quantitative RESULTS: of 
      the two genes expression were analysed along with the stages of hepatic fibrosis. 
      RESULTS: Typical liver fibrosis developed in the model group rats, and the 
      positive stain of CTGF mRNA and TGFbeta1 mRNA increased, which was distributed in 
      the areas where fibrosis occurred. There was obvious correlation between the 
      expression strength of CTGF mRNA and TGFbeta1 mRNA (r = 0.799, P < 0.05). In the 
      rats receiving HanDanGanLe, CTGF mRNA expression index decreased markedly 
      (12.5+/-2.3 vs 28.8+/-1.4, t = 5.208, P < 0.01), so did TGFbeta1 mRNA expression 
      index (25.4+/-3.2 vs 37.3+/-5.4, t = 5.655, P < 0.01). There was also significant 
      correlation between the scores of CTGF mRNA expression and the stages of hepatic 
      fibrosis (rs = 0.822, 0.808 in the model group and HanDanGanLe-treatment group, P 
      < 0.05). CONCLUSIONS: The expression of CTGF mRNA and TGFbeta1 mRNA is correlated 
      closely with hepatic fibrosis degree. HanDanGanLe can effectively prevent the 
      expression of CTGF and TGFbeta1. One of the mechanisms of the intervention may be 
      its blocking the intracellular signalling pathways involved in liver 
      fibrogenesis.
FAU - Zhang, Wen-sheng
AU  - Zhang WS
AD  - Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical 
      College, Guiyang 550004, China.
FAU - Cheng, Ming-liang
AU  - Cheng ML
FAU - Lu, Yin-ying
AU  - Lu YY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (CCN2 protein, rat)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Animals
MH  - Connective Tissue Growth Factor
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Immediate-Early Proteins/*biosynthesis/genetics
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis/genetics
MH  - Liver Cirrhosis, Experimental/chemically induced/drug therapy/*metabolism
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Transforming Growth Factor beta/*biosynthesis/genetics
MH  - Transforming Growth Factor beta1
EDAT- 2003/05/30 05:00
MHDA- 2005/03/11 09:00
CRDT- 2003/05/30 05:00
PHST- 2003/05/30 05:00 [pubmed]
PHST- 2005/03/11 09:00 [medline]
PHST- 2003/05/30 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2003 May;11(5):285-7.