PMID- 12775230
OWN - NLM
STAT- MEDLINE
DCOM- 20030612
LR  - 20131121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 116
IP  - 2
DP  - 2003 Feb
TI  - Effect of preoperative transcatheter arterial chemoembolization on apoptosis of 
      hepatocellular carcinoma cells.
PG  - 203-7
AB  - OBJECTIVE: To evaluate the effect of preoperative transcatheter arterial 
      chemoembolization(TACE) on apoptosis of hepatocellular carcinoma (HCC) cells. 
      METHODS: A total of 136 patients with HCC underwent liver resection. One to five 
      courses of TACE prior to liver resection were performed in 79 patients (TACE 
      group), in which one to four courses of chemotherapy alone were performed in 11 
      patients (group A), one to five courses of chemotherapy combined with iodized oil 
      were performed in 33 patients (group B), one to three courses of chemotherapy 
      combined with iodized oil and gelatin sponge were performed in 23 patients group 
      C) and one to three courses of chemotherapy combined with iodized oil, ethanol 
      and gelatin sponge were performed in 12 patients (group D). The other 57 patients 
      only received liver resection (non-TACE group). The extent of apoptosis was 
      analyzed by transferase-mediated dUTP nick end labeling (TUNEL) staining. The 
      expressions of Bcl-2 and Bax protein were detected by immunohistochemical method. 
      RESULTS: The apoptotic index(AI) and level of Bax protein in HCC cells were 
      significantly higher in groups A, B, C and D than those in the non-TACE group (P 
      < 0.05). The level of Bcl-2 protein and ratio of Bcl-2 to Bax protein of HCC 
      cells were significantly lower in Groups A, B, C and D than those in the non-TACE 
      group (P < 0.05). CONCLUSION: Preoperative TACE regimens may enhance apoptosis of 
      HCC cells by up-regulating the expression of Bax protein and down-regulating the 
      expression of Bcl-2 protein and ratio of Bcl-2 to Bax protein expression.
FAU - Xiao, Enhua
AU  - Xiao E
AD  - Department of Radiology, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China. xiaogdk@sohu.com
FAU - Li, Detai
AU  - Li D
FAU - Shen, Shubin
AU  - Shen S
FAU - Zhou, Shunke
AU  - Zhou S
FAU - Tan, Lihua
AU  - Tan L
FAU - Wang, Yunhua
AU  - Wang Y
FAU - Luo, Jianguang
AU  - Luo J
FAU - Wu, Yuzhi
AU  - Wu Y
FAU - Tan, Changlian
AU  - Tan C
FAU - Liu, Hui
AU  - Liu H
FAU - Zhu, Hui
AU  - Zhu H
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (BAX protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 3K9958V90M (Ethanol)
RN  - 8001-40-9 (Iodized Oil)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Apoptosis
MH  - Carcinoma, Hepatocellular/chemistry/pathology/*therapy
MH  - *Chemoembolization, Therapeutic
MH  - Ethanol/administration & dosage
MH  - Female
MH  - Humans
MH  - Iodized Oil/administration & dosage
MH  - Liver Neoplasms/chemistry/pathology/*therapy
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins/analysis
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - bcl-2-Associated X Protein
EDAT- 2003/05/31 05:00
MHDA- 2003/06/13 05:00
CRDT- 2003/05/31 05:00
PHST- 2003/05/31 05:00 [pubmed]
PHST- 2003/06/13 05:00 [medline]
PHST- 2003/05/31 05:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2003 Feb;116(2):203-7.