PMID- 12775237
OWN - NLM
STAT- MEDLINE
DCOM- 20030612
LR  - 20071115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 116
IP  - 2
DP  - 2003 Feb
TI  - Clinical, cytogenetic and dual-color FISH studies on five cases of 
      myelodysplastic syndrome or acute myeloid leukemia patients with 1;7 
      translocation.
PG  - 231-4
AB  - OBJECTIVE: To study the clinical and cytogenetic characteristics of four patients 
      with myelodysplastic syndrome (MDS) and one with acute myeloid leukemia 
      experiencing t (1;7). METHODS: Five patients seen in our hospital from 1992 to 
      2001 were diagnosed as MDS and acute myelocytic leukemia (AML) according to the 
      French-American-British (FAB) criteria. Chromosomes were prepared using the 
      direct method as well as 24-hour unstimulated cultures of fresh heparinized bone 
      marrow for each subject, while R-banding was used to analyze karyotypes. 
      Dual-color fluorescence in situ hybridization (FISH) using SpectrumRed and 
      SpectrumGreen directly labeled chromosome 1-specific alpha-satellite DNA probe 
      (red) and chromosome 7- specific alpha-satellite DNA probe (green) was performed 
      for three cases. RESULTS: Of the five patients, three had 1;7 translocation due 
      to a long history of exposure to benzene. In three cases, dual-color FISH 
      resulted in three red signals and two green ones, in which one red signal 
      adjoining one green signal in 27.6%, 84% and 18.5% metaphases, respectively. 
      CONCLUSIONS: Exposure to benzene may be the cause for Chinese MDS and AML 
      patients with t (1;7) translocation. The result of dual-color FISH convincingly 
      confirmed that the centromere of the derivative chromosome 7p/1q resulting from 
      1;7 translocation was made up of centromeres from both chromosomes 1 and 7.
FAU - Shen, Yongmei
AU  - Shen Y
AD  - Leukemia Research Unit, Jiangsu Institution of Hematology, First Affiliated 
      Hospital of Soochow University, Suzhou 215006, China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Li, Jianyong
AU  - Li J
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wu, Yafang
AU  - Wu Y
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Adult
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Myelodysplastic Syndromes/*genetics
MH  - *Translocation, Genetic
EDAT- 2003/05/31 05:00
MHDA- 2003/06/13 05:00
CRDT- 2003/05/31 05:00
PHST- 2003/05/31 05:00 [pubmed]
PHST- 2003/06/13 05:00 [medline]
PHST- 2003/05/31 05:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2003 Feb;116(2):231-4.