PMID- 12775514
OWN - NLM
STAT- MEDLINE
DCOM- 20030701
LR  - 20181130
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 66
IP  - 11
DP  - 2003 Jun 13
TI  - Asphalt exposure enhances neuropeptide levels in sensory neurons projecting to 
      the rat nasal epithelium.
PG  - 1015-27
AB  - Asphalt fumes have been reported to produce nasal irritation in road workers. 
      Since inhaled irritants can increase substance P (SP) production in airway 
      neurons, the effects of asphalt fumes on SP production in trigeminal ganglia (TG) 
      sensory neurons innervating the nasal mucosa were investigated. The effects of 
      asphalt fumes on nasal mucosal innervation were examined by measuring SP and 
      calcitonin-gene-related peptide (CGRP) levels in rat TG neurons projecting to the 
      nasal epithelium. Female Sprague-Dawley rats were exposed to asphalt fumes at 
      16.0 +/- 8.1mg /m3 for 5 consecutive days, 3.5 h/d. Inflammatory cells were 
      measured in nasal cavity lavage fluid. SP and CGRP immunoreactivity (IR) was 
      measured in the cell bodies of trigeminal ganglion sensory neurons projecting to 
      the nasal cavity. A significant increase in neutrophils and macrophages was 
      observed after asphalt fume exposure indicating an inflammatory response in the 
      nasal cavity. The percentage of SP-IR neurons increased significantly in the 
      asphalt-exposed rats, and the proportion of CGRP-IR neurons was also elevated 
      following asphalt exposure. These results indicate that exposure to asphalt fumes 
      produces inflammation and increases the levels of SP and CGRP in TG neurons 
      projecting to the nasal epithelium. The findings are consistent with 
      asphalt-induced activation of sensory C-fibers in the nasal cavity. Enhanced 
      sensory neuropeptide release from nerve terminals in the nasal cavity may produce 
      neurogenic inflammation associated with nasal irritation following exposure to 
      asphalt fumes.
FAU - Sikora, Erin R
AU  - Sikora ER
AD  - Department of Neurobiology, West Virginia University, Morgantown, West Virginia 
      26506-9128, USA.
FAU - Stone, Sam
AU  - Stone S
FAU - Tomblyn, Seth
AU  - Tomblyn S
FAU - Frazer, Dave G
AU  - Frazer DG
FAU - Castranova, Vincent
AU  - Castranova V
FAU - Dey, Richard D
AU  - Dey RD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Hydrocarbons)
RN  - 0 (Rhodamines)
RN  - 33507-63-0 (Substance P)
RN  - 8052-42-4 (asphalt)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/*metabolism
MH  - Epithelium/innervation/metabolism
MH  - Female
MH  - Hydrocarbons/*toxicity
MH  - Immunohistochemistry
MH  - Inflammation
MH  - Inhalation Exposure
MH  - Microspheres
MH  - Nasal Mucosa/*innervation/*metabolism
MH  - Neurons/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rhodamines
MH  - Substance P/*metabolism
MH  - Therapeutic Irrigation
EDAT- 2003/05/31 05:00
MHDA- 2003/07/02 05:00
CRDT- 2003/05/31 05:00
PHST- 2003/05/31 05:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2003/05/31 05:00 [entrez]
AID - ENNUM1J4WE2GQAF0 [pii]
AID - 10.1080/15287390306394 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2003 Jun 13;66(11):1015-27. doi: 
      10.1080/15287390306394.