PMID- 12776605
OWN - NLM
STAT- MEDLINE
DCOM- 20030922
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 30
IP  - 2
DP  - 2003 Feb
TI  - [Cloning and expression of maize fructose-6-phosphate, 
      2-kinase/fructose-2,6-bisphosphatase (mF2KP)].
PG  - 163-8
AB  - A 2469 bp cDNA encoding entire fructose-6-phosphate, 
      2-kinase/fructose-2,6-bisphosphatase (F2KP) was cloned from maize (Zea mays L.cv. 
      Ziyunuo 1) by the methods of RT-PCR and rapid amplification of cDNA ends(RACE), 
      on the basis of AF007582, which was isolated from "Yedan 4". The cDNA was 
      designated as mF2KP and the GenBank accession number is AF334143, which contains 
      a 2226 bp open reading from (ORF), encoding a 741 residue polypeptide. There are 
      some differences between the F2KP genes of two maize varieties. The length of 3' 
      non-coding region of mF2KP is 38 bp shorter than that of AF007582. On the 1592th, 
      1593th and 1605th positions of mF2KP, there is an additional nucleotide 
      respectively compared with AF007582, which cause a shifted reading frame in a 
      small region. Northern blot showed that the expression of mF2KP were 
      significantly different among maize tissues. The Transcription of mF2KP in stem 
      was lower than those in leaves, kernel leaves and male inflorescence, but much 
      higher than that in immature seeds.
FAU - Wang, Dong
AU  - Wang D
AD  - National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 
      Agricultural University, Nanjing 210095, China.
FAU - Du, Xi-Ling
AU  - Du XL
FAU - Hu, Jian-Guang
AU  - Hu JG
FAU - Zhang, Hong-Sheng
AU  - Zhang HS
FAU - Yang, Jin-Shui
AU  - Yang JS
FAU - Zhai, Hu-Qu
AU  - Zhai HQ
LA  - chi
SI  - GENBANK/AF334143
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Plant)
RN  - EC 2.7.1.105 (Phosphofructokinase-2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - Molecular Sequence Data
MH  - Phosphofructokinase-2/*genetics
MH  - RNA, Plant/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Zea mays/enzymology/*genetics
EDAT- 2003/06/05 05:00
MHDA- 2003/09/23 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/09/23 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2003 Feb;30(2):163-8.