PMID- 12778743
OWN - NLM
STAT- MEDLINE
DCOM- 20040419
LR  - 20121115
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 38
IP  - 2
DP  - 2003 Feb
TI  - [Protective effect of puerarin on vascular endothelial cell apoptosis induced by 
      chemical hypoxia in vitro].
PG  - 103-7
AB  - AIM: To study the effect of puerarin on vascular endothelial cells apoptosis 
      induced by chemical hypoxia-ischemia in vitro. METHODS: The chemical 
      hypoxia-ischemia model was performed by treating cultured bovine aortic 
      endothelial cells (BAECs) with NaCN in glucose-free medium. Cell viability was 
      determined by trypan blue staining. Cell apoptosis was defined by terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), flow 
      cytometry and Hoechst 33342 staining. The expression of Caspase-3 in endothelial 
      cells was detected by immunocytochemical method. RESULTS: Chemical 
      hypoxia-ischemia was shown to initiate bovine aortic endothelial cell apoptosis. 
      Puerarin (0.5-3 mmol.L-1) was found to inhibit endothelial cell apoptosis 
      effectively, and reduce the expression of Caspase-3 significantly. CONCLUSION: 
      Puerarin can protect apoptotic endothelial cells induced by chemical 
      hypoxia-ischemia markedly and the effect was performed partly by decreasing 
      Caspase-3 expression.
FAU - Shi, Rui-li
AU  - Shi RL
AD  - Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Beijing 100050, China.
FAU - Zhang, Jian-jun
AU  - Zhang JJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Isoflavones)
RN  - 0 (Protective Agents)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/cytology
MH  - *Apoptosis
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Cattle
MH  - Cell Hypoxia/drug effects
MH  - Cells, Cultured
MH  - DNA Damage/drug effects
MH  - Endothelium, Vascular/cytology/*drug effects
MH  - Isoflavones/*pharmacology
MH  - Protective Agents/*pharmacology
EDAT- 2003/06/05 05:00
MHDA- 2004/04/20 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2004/04/20 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
PST - ppublish
SO  - Yao Xue Xue Bao. 2003 Feb;38(2):103-7.