PMID- 12781444
OWN - NLM
STAT- MEDLINE
DCOM- 20030627
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 143
IP  - 2
DP  - 2003 Jun
TI  - The effect of chlorambucil treatment on cytogenetic parameters in chronic 
      lymphocytic leukemia patients.
PG  - 113-9
AB  - The most common treatment of chronic lymphocytic leukemia (CLL) is the alkylating 
      agent chlorambucil (CLB), with or without prednisone. In the present study, our 
      aim was to evaluate whether treatment with CLB for more than one year induced 
      genetic changes manifested by comparative genomic hybridization (CGH) as new 
      chromosomal aberrations. We also studied whether CLB affected the pattern of 
      replication by using fluorescence in situ hybridization (FISH). We found a 
      similar rate of asynchronous pattern of replication in both treated and untreated 
      patients with CLL. Most of the aberrations found with CGH were previously 
      reported in CLL. More prognostically unfavorable aberrations and more cases with 
      genetic changes were found in the treated group. The changes found were not 
      typical of the secondary genetic aberrations associated with alkylating agents. 
      Thus, we conclude that treatment of CLL with CLB for at least a year does not 
      affect the parameters analyzed in this study. Longer studies are needed to 
      further explore the effects of alkylating agents on normal and malignant cells.
FAU - Amiel, A
AU  - Amiel A
AD  - Genetic Institute, Meir Hospital, Kfar-Saba, Israel. AlizaAmiel@clalit.org.il
FAU - Biton, I
AU  - Biton I
FAU - Yukla, M
AU  - Yukla M
FAU - Gaber, E
AU  - Gaber E
FAU - Fejgin, M D
AU  - Fejgin MD
FAU - Lishner, M
AU  - Lishner M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Antineoplastic Agents, Alkylating)
RN  - 18D0SL7309 (Chlorambucil)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antineoplastic Agents, Alkylating/*pharmacology/therapeutic use
MH  - Chlorambucil/*pharmacology/therapeutic use
MH  - Chromosome Aberrations/*drug effects
MH  - Chromosomes, Human, Pair 12/genetics
MH  - DNA Replication/drug effects
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy/*genetics
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - Time Factors
MH  - Trisomy/genetics
EDAT- 2003/06/05 05:00
MHDA- 2003/06/28 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/06/28 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
AID - S016546080200852X [pii]
AID - 10.1016/s0165-4608(02)00852-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Jun;143(2):113-9. doi: 
      10.1016/s0165-4608(02)00852-x.