PMID- 12782009
OWN - NLM
STAT- MEDLINE
DCOM- 20030701
LR  - 20190910
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 74
IP  - 3
DP  - 2003 Jun
TI  - Vascular expression of matrix metalloproteinase-13 (collagenase-3) in basal cell 
      carcinoma.
PG  - 230-7
AB  - Matrix metalloproteinase-13 (MMP-13; collagenase-3) was detected in the 
      vasculature from 17 of 20 human basal cell carcinomas as assessed by 
      immunohistology immediately after surgery. In contrast, MMP-1 (interstitial 
      collagenase) was detected in the vasculature of only two of the same specimens. 
      MMP-13 reactivity was also observed in the capillaries of normal human skin taken 
      from the wound margin. Human dermal microvascular endothelial cells as well as 
      human umbilical vein endothelial cells were isolated in culture and examined for 
      MMP-13 expression. By reverse transcription-polymerase chain reaction and 
      Southern blotting, an MMP-13 transcript was detected in unstimulated endothelial 
      cells. The transcript was upregulated in cells treated with 50 nM phorbol 
      myristate acetate (PMA). Western blotting demonstrated the presence of an 
      anti-MMP-13 - immunoreactive protein in culture fluid from both cell sources. 
      Immunoreactivity was stronger in culture fluid from cells treated with 
      interleukin-1alpha (IL-1alpha) than in culture fluid from control cells. Tumor 
      necrosis factor-alpha (TNF-alpha) and PMA also upregulated MMP-13 expression but 
      these agents were not as effective as IL-1alpha. Additionally, reactivity was 
      greater in culture fluid from endothelial cells grown on three-dimensional 
      lattices of polymerized type I collagen than on dried collagen films. These data 
      indicate that endothelial cells in the skin are a source of MMP-13 and that 
      enzyme expression is upregulated under conditions that promote endothelial cell 
      growth and vascular differentiation.
FAU - Hattori, Yukari
AU  - Hattori Y
AD  - Department of Pathology, The University of Michigan, Ann Arbor 48109, USA.
FAU - Nerusu, Kamalakar C
AU  - Nerusu KC
FAU - Bhagavathula, Narasimharao
AU  - Bhagavathula N
FAU - Brennan, Meghan
AU  - Brennan M
FAU - Hattori, Noboru
AU  - Hattori N
FAU - Murphy, Hedwig S
AU  - Murphy HS
FAU - Su, Lyndon D
AU  - Su LD
FAU - Wang, Timothy S
AU  - Wang TS
FAU - Johnson, Timothy M
AU  - Johnson TM
FAU - Varani, James
AU  - Varani J
LA  - eng
GR  - CA60958/CA/NCI NIH HHS/United States
GR  - DK59169/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (MMP13 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Capillaries/chemistry/enzymology/pathology
MH  - Carcinoma, Basal Cell/*blood supply/pathology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Collagenases/genetics/*metabolism
MH  - Culture Media, Conditioned/chemistry
MH  - Dermis/blood supply
MH  - Endothelium, Vascular/drug effects/*enzymology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Matrix Metalloproteinase 13
MH  - Organ Culture Techniques
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Skin Neoplasms/*blood supply/pathology/surgery
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2003/06/05 05:00
MHDA- 2003/07/02 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
AID - S0014480003000145 [pii]
AID - 10.1016/s0014-4800(03)00014-5 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2003 Jun;74(3):230-7. doi: 10.1016/s0014-4800(03)00014-5.