PMID- 12783130
OWN - NLM
STAT- MEDLINE
DCOM- 20031002
LR  - 20161013
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 102
IP  - 3
DP  - 2003 Mar
TI  - Association between levels of TNF-alpha and TNF-alpha promoter -308 A/A 
      polymorphism in children with Kawasaki disease.
PG  - 147-50
AB  - BACKGROUND AND PURPOSE: Tumor necrosis factor-alpha (TNF-alpha) has been shown to 
      play a central role in the pathogenesis of vasculitis in Kawasaki disease (KD). 
      We investigated the serum levels of TNF-alpha and soluble TNF receptor 1 (STNFR1) 
      levels, and genetic polymorphisms of the TNF-alpha promoter gene in children with 
      KD to delineate the genetic basis of KD. METHODS: A total of 18 children (12 boys 
      and 6 girls) with KD were studied, 9 of whom had the complication of coronary 
      artery lesion (CAL) within 30 days after the onset of symptoms. Serum levels of 
      TNF-alpha and STNFR1 were assayed by enzyme-linked immnosorbent assay, and DNA 
      polymorphisms of the 5' flanking region of TNF-alpha promoter gene at position 
      -308 [guanine (G) to adenine (A)] and -238 (G to A) were studied by direct 
      nucleotide sequencing. RESULTS: The serum TNF-alpha level in KD patients was 113 
      +/- 209.9 pg/mL (range, 2.0 to 756.9 pg/mL; median, 24.7 pg/mL; normal, < 10 
      pg/mL). The serum levels of STNFR1 in KD (4255 +/- 2425 pg/mL) were higher than 
      those of the control group (160 +/- 116 pg/mL). Allele frequencies of -308A and 
      -238A were 11.1% and 0% in the KD patients, and 0% and 3.1% in the control group. 
      Neither TNF-alpha promoter polymorphism nor any significant risk factor for CAL 
      was identified in KD patients. One patient, who was homozygous for -308A, showed 
      the highest TNF-alpha level and elevated STNFR1 level but had no evidence of CAL. 
      Positive correlations were found between serum levels of STNFR1 and C-reactive 
      protein (r = 0.731, p = 0.007), and between STNFR1 and leukocyte counts at 
      admission (r = 0.620, p = 0.008). CONCLUSIONS: Increased serum levels of 
      TNF-alpha and STNFR1 were found in KD patients but there was no correlation 
      between these levels. The relationship between the pathogenesis of KD and 
      TNF-alpha gene promoter -308G to A mutation towards cytokine production remains 
      to be clarified.
FAU - Chien, Yin-Hsiu
AU  - Chien YH
AD  - Department of Pediatrics, National Taiwan University Hospital and National Taiwan 
      University, Taipei, Taiwan.
FAU - Chang, Kuei-Wen
AU  - Chang KW
FAU - Yang, Yao-Hsu
AU  - Yang YH
FAU - Lu, Meng-Yao
AU  - Lu MY
FAU - Lin, Yu-Tsan
AU  - Lin YT
FAU - Chiang, Bor-Luen
AU  - Chiang BL
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Frequency
MH  - Humans
MH  - Infant
MH  - Male
MH  - Mucocutaneous Lymph Node Syndrome/*genetics
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic/genetics
MH  - Receptors, Tumor Necrosis Factor/blood/*genetics
MH  - Tumor Necrosis Factor-alpha/analysis/*genetics
EDAT- 2003/06/05 05:00
MHDA- 2003/10/03 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/10/03 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
PST - ppublish
SO  - J Formos Med Assoc. 2003 Mar;102(3):147-50.