PMID- 12783131
OWN - NLM
STAT- MEDLINE
DCOM- 20031002
LR  - 20161013
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 102
IP  - 3
DP  - 2003 Mar
TI  - Vitamin C protects against lysophosphatidylcholine-induced expression of monocyte 
      chemoattractant protein-1 in cultured human umbilical vein endothelial cells.
PG  - 151-7
AB  - BACKGROUND AND PURPOSE: It is well documented that oxidized low-density 
      lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce 
      monocyte chemoattractant protein-1 (MCP-1). Vitamin C is known to be an important 
      antioxidant for vasodilatation. The purpose of this study was to determine 
      whether pretreatment with vitamin C could protect against oxidized-LDL-induced 
      expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). 
      METHODS: Cultured HUVECs were used for desired experiments before passage 4. 
      Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as 
      the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in 
      the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 
      RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain 
      reaction. RESULTS: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 
      microM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with 
      vitamin C in concentrations of 50, 100, 150, and 200 microM, reduced levels of 
      MCP-1 in the culture medium by 44%, 51%, 60%, and 67%, respectively, while levels 
      of MCP-1 mRNA decreased by 15%, 18%, 80%, and 82%, respectively. CONCLUSIONS: Our 
      findings imply that pretreatment with vitamin C can suppress lysoPC-induced 
      expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is 
      protective against lysoPC-mediated inflammatory insults to the vascular 
      endothelium in vitro.
FAU - Pan, Ju-Pin
AU  - Pan JP
AD  - Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, 
      School of Medicine, National Yang-Ming University, Taipei, Taiwan.
FAU - Cheng, Tsai-Mu
AU  - Cheng TM
FAU - Chou, Shiu-Chin
AU  - Chou SC
FAU - Lai, Shiau-Ting
AU  - Lai ST
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
RN  - 0 (Chemokine CCL2)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (RNA, Messenger)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Ascorbic Acid/*pharmacology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Free Radical Scavengers/*pharmacology
MH  - Humans
MH  - Lysophosphatidylcholines/*antagonists & inhibitors/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Umbilical Veins/cytology/metabolism
EDAT- 2003/06/05 05:00
MHDA- 2003/10/03 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/10/03 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
PST - ppublish
SO  - J Formos Med Assoc. 2003 Mar;102(3):151-7.