PMID- 12784246
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20071114
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 65
IP  - 3
DP  - 2003 Jul
TI  - Localization of glutamate cysteine ligase subunit mRNA within the rat ovary and 
      relationship to follicular apoptosis.
PG  - 254-61
AB  - Ovarian levels of the antioxidant tripeptide glutathione (GSH) increase following 
      gonadotropin administration, suggesting that GSH synthesis in the ovary may be 
      associated with follicular growth. In situ hybridization with (35)S-labeled 
      riboprobes was used to localize ovarian mRNA expression of the catalytic and 
      modulatory subunits of glutamate cysteine ligase (Gclc and Gclm), the 
      rate-limiting enzyme in GSH synthesis, during each stage of the rat estrous 
      cycle. Gclm was highly expressed in the granulosa cells and oocytes of healthy, 
      growing follicles, not in atretic follicles. Gclc was also highly expressed in 
      follicles; however, unlike Gclm, Gclc was also expressed in corpora lutea and 
      interstitial cells. In a subsequent experiment, the hypothesis that GSH synthesis 
      occurs in healthy, but not in apoptotic, follicles was tested. Terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to 
      detect apoptotic cells in the ovaries, and in situ hybridization for Gclm and 
      Gclc was performed in adjacent sections of the same ovaries. TUNEL staining was 
      found to be significantly associated with absence of Gclm hybridization in 
      granulosa cells and oocytes and with lack of strong Gclc hybridization in 
      granulosa cells. These results suggest that follicular apoptosis may be 
      associated with down-regulation of Gclm and Gclc transcription in granulosa cells 
      and oocytes.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Luderer, Ulrike
AU  - Luderer U
AD  - Department of Medicine, Division of Occupational and Environmental Medicine, 
      University of California, Irvine, California 96212, USA. uluderer@uci.edu
FAU - Diaz, Dolores
AU  - Diaz D
FAU - Faustman, Elaine M
AU  - Faustman EM
FAU - Kavanagh, Terrance J
AU  - Kavanagh TJ
LA  - eng
GR  - 1 K08 ES-10963/ES/NIEHS NIH HHS/United States
GR  - 1 P30 ES07033-03/ES/NIEHS NIH HHS/United States
GR  - 1 P42 ES-04696-14001/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Protein Subunits)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Antisense)
RN  - 0 (RNA, Messenger)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Glutamate-Cysteine Ligase/*genetics
MH  - In Situ Hybridization
MH  - In Situ Nick-End Labeling
MH  - Ovarian Follicle/*cytology/physiology
MH  - Ovary/*enzymology
MH  - Protein Subunits/genetics
MH  - RNA Probes
MH  - RNA, Antisense
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription, Genetic
EDAT- 2003/06/05 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
AID - 10.1002/mrd.10298 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2003 Jul;65(3):254-61. doi: 10.1002/mrd.10298.