PMID- 12784397
OWN - NLM
STAT- MEDLINE
DCOM- 20031007
LR  - 20041117
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 30
IP  - 6
DP  - 2003 Jun
TI  - Spontaneous chromosome damage (micronuclei) in systemic sclerosis and Raynaud's 
      phenomenon.
PG  - 1244-7
AB  - OBJECTIVE: To evaluate the prevalence of spontaneous chromosome damage in 
      cultured peripheral lymphocytes of patients with systemic sclerosis (SSc), 
      idiopathic Raynaud's phenomenon (RP), and suspected secondary RP, by means of 
      molecular cytogenetic analysis. METHODS: We studied 43 patients with SSc, 13 with 
      idiopathic RP, and 16 with suspected secondary RP and 25 healthy controls. As a 
      marker of chromosome alteration we used the micronucleus (MN) assay. All subjects 
      were also classified for antinuclear antibodies, anticentromere antibodies (ACA), 
      or Scl70. To identify the mechanism of MN formation, we also performed MN 
      fluorescence in situ hybridization (FISH) analysis using a pancentromeric DNA 
      probe. RESULTS: Patients with SSc and subjects with RP showed significantly 
      higher MN frequencies than controls (25.9 +/- 1.7 and 19.1 +/- 2.15, 
      respectively, vs 9.4 +/- 2.2; p < 0.001). Subjects with suspected secondary RP 
      displayed MN frequency (23.5 +/- 2.7) comparable to that of SSc patients, while 
      spontaneous MN level in idiopathic RP subjects (13.6 +/- 3.0) did not differ 
      significantly from controls (9.4 +/- 2.2). ACA positive subjects showed the 
      highest MN frequencies (32.8 +/- 1.7) compared to subjects with a different 
      antibody pattern (18.3 +/- 1.6). CONCLUSION: Our results show the presence of 
      higher levels of micronuclei in circulating lymphocytes of patients with SSc and 
      subjects with suspected secondary RP. They also suggest a possible role of ACA in 
      determining cytogenetic anomalies. FISH analysis indicated that both 
      aneuploidogenic and clastogenic events contributed to the formation of MN 
      observed in SSc patients and subjects with suspected secondary RP.
FAU - Porciello, Giovanni
AU  - Porciello G
AD  - Istituto di Reumatologia, University of Siena, Italy. g.porciello@katamail.com
FAU - Scarpato, Roberto
AU  - Scarpato R
FAU - Ferri, Clodoveo
AU  - Ferri C
FAU - Storino, Franca
AU  - Storino F
FAU - Cagetti, Francesca
AU  - Cagetti F
FAU - Morozzi, Gabriella
AU  - Morozzi G
FAU - Bellisai, Francesca
AU  - Bellisai F
FAU - Migliore, Lucia
AU  - Migliore L
FAU - Marcolongo, Roberto
AU  - Marcolongo R
FAU - Galeazzi, Mauro
AU  - Galeazzi M
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Autoantibodies)
SB  - IM
MH  - Adult
MH  - Autoantibodies/blood
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Micronuclei, Chromosome-Defective
MH  - Middle Aged
MH  - Raynaud Disease/*genetics/immunology
MH  - Scleroderma, Systemic/*genetics/immunology
EDAT- 2003/06/05 05:00
MHDA- 2003/10/08 05:00
CRDT- 2003/06/05 05:00
PHST- 2003/06/05 05:00 [pubmed]
PHST- 2003/10/08 05:00 [medline]
PHST- 2003/06/05 05:00 [entrez]
AID - 0315162X-30-1244 [pii]
PST - ppublish
SO  - J Rheumatol. 2003 Jun;30(6):1244-7.