PMID- 12785589
OWN - NLM
STAT- MEDLINE
DCOM- 20031009
LR  - 20161018
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 22
IP  - 6
DP  - 2003 Jun
TI  - The relationships of biochemical endpoints to histopathology and population 
      metrics in feral flatfish species collected near the municipal wastewater outfall 
      of Orange County, California, USA.
PG  - 1309-17
AB  - In July 2000, 330 individuals of three flatfish species were collected from 
      reference locations and nine sites surrounding the outfall of the Orange County 
      (CA, USA) Sanitation District (OCSD) municipal wastewater discharge. Species 
      included hornyhead turbot (Pleuronichthys verticalis), English sole (Pleuronectes 
      vetulus), and bigmouth sole (Hippoglossina stomata). Livers of sampled animals 
      were examined for the expression of vitellogenin (in males), cytochrome P450 1A 
      (CYP1A), and DNA damage (comet assay). Biliary fluorescent aromatic compounds 
      (FACs) were also measured. Comparisons with tissue and sediment contaminant 
      residues, liver histopathology, and population estimates were assessed to 
      determine whether relationships exist between levels of biological organization. 
      The CYP1A results indicated exposure to planar aromatic hydrocarbons at one 
      nearshore site. Regression analysis of all English sole CYP1A to total sediment 
      polychlorinated biphenyls (PCBs) (r2 = 0.97; p < 0.05) indicated a significant 
      correlation. Widespread exposure to estrogens was observed at all sites without 
      correlation to the abundance of the three species examined. Fluorescent aromatic 
      compounds demonstrated no correlation to CYP1A expression. Overall, 
      histopathology and biochemical endpoints did not indicate significant adverse 
      effects in fish at the OCSD outfall.
FAU - Roy, Luke A
AU  - Roy LA
AD  - Department of Environmental Sciences, University of California, Riverside, 
      California 92521, USA.
FAU - Armstrong, Jeffrey L
AU  - Armstrong JL
FAU - Sakamoto, Ken
AU  - Sakamoto K
FAU - Steinert, Scott
AU  - Steinert S
FAU - Perkins, Edwin
AU  - Perkins E
FAU - Lomax, Daniel P
AU  - Lomax DP
FAU - Johnson, Lyndal L
AU  - Johnson LL
FAU - Schlenk, Daniel
AU  - Schlenk D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Biomarkers)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (Vitellogenins)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - California
MH  - Cytochrome P-450 Enzyme System/analysis/biosynthesis
MH  - DNA Damage
MH  - Ecology
MH  - Environmental Monitoring/*methods
MH  - Female
MH  - Flatfishes/classification/growth & development/*physiology
MH  - Fluorescent Dyes/chemistry
MH  - Geologic Sediments/analysis/chemistry
MH  - Liver/drug effects/metabolism/*pathology
MH  - Male
MH  - Polycyclic Compounds/chemistry/toxicity
MH  - Vitellogenins/analysis/biosynthesis
MH  - *Waste Disposal, Fluid
EDAT- 2003/06/06 05:00
MHDA- 2003/10/10 05:00
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2003/10/10 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
PST - ppublish
SO  - Environ Toxicol Chem. 2003 Jun;22(6):1309-17.