PMID- 12787392
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20071114
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 64
IP  - 1
DP  - 2003 Jul
TI  - Altered expression of immune modulator and structural genes in neonatal 
      unilateral ureteral obstruction.
PG  - 25-35
AB  - BACKGROUND: Congenital obstructive nephropathy is a condition characterized by 
      hydronephrosis, tubular dilatation, apoptosis, and atrophy, as well as 
      interstitial cellular infiltration and progressive interstitial fibrosis. The 
      renal consequences of chronic unilateral ureteral obstruction (UUO) in the 
      neonatal rat are similar to those of clinical congenital obstructive nephropathy. 
      METHODS: To define alterations in renal gene expression induced by chronic 
      neonatal UUO, Sprague-Dawley rats were subjected to UUO or sham operation within 
      the first 2 days of life, and kidneys were harvested after 12 days. RESULTS: 
      Microarray analysis revealed that the mRNA expression of multiple immune 
      modulators, including krox24, interferon-gamma regulating factor-1 (IRF-1), 
      monocyte chemoattractant protein-1 (MCP-1), interleukin-1beta (IL-1beta), 
      CCAAT/enhancer binding protein (C/EBP), p21, c-fos, c-jun, and pJunB, was 
      significantly increased in obstructed compared to sham-operated kidneys (all P < 
      0.05). Western blot analysis revealed significant changes in immune modulator 
      protein abundance in the obstructed versus sham-operated kidney for krox24 (P = 
      0.0004), IRF-1 (P = 0.005), MCP-1 (P = 0.01), and JunD (P = 0.0008). 
      Alternatively, the abundance of all of the immune modulator proteins was similar 
      in sham-operated and obstructed kidneys in rats subjected to acute (4 days) 
      neonatal UUO. Microarray analysis studies also reveal that structural genes that 
      comprise the cytoskeleton and cell matrix are significantly up-regulated by 
      chronic neonatal UUO, including calponin, desmin, dynamin, and lumican (all P < 
      0.05). CONCLUSION: Multiple genes are aberrantly expressed in the kidney of rats 
      subjected to chronic neonatal UUO. Elucidation of these genes involved in 
      neonatal UUO may lead to new insight about congenital obstructive nephropathy.
FAU - Silverstein, Douglas M
AU  - Silverstein DM
AD  - Division of Nephrology, Department of Pediatrics, Gene Therapy Program, Louisiana 
      State University Health Sciences Center, New Orleans, Louisiana 70124, USA. 
      dsilve@lsuhsc.edu
FAU - Travis, Brett R
AU  - Travis BR
FAU - Thornhill, Barbara A
AU  - Thornhill BA
FAU - Schurr, Jill S
AU  - Schurr JS
FAU - Kolls, Jay K
AU  - Kolls JK
FAU - Leung, Jocelyn C
AU  - Leung JC
FAU - Chevalier, Robert L
AU  - Chevalier RL
LA  - eng
GR  - DK45179/DK/NIDDK NIH HHS/United States
GR  - DK52612/DK/NIDDK NIH HHS/United States
GR  - HD28810/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Kidney Int. 2003 Jul;64(1):361-2. PMID: 12787430
MH  - Adjuvants, Immunologic/genetics/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Chronic Disease
MH  - Cytoskeleton/genetics
MH  - *Gene Expression
MH  - *Genes
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
MH  - Ureteral Obstruction/*genetics/*metabolism
EDAT- 2003/06/06 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
AID - S0085-2538(15)49289-0 [pii]
AID - 10.1046/j.1523-1755.2003.00067.x [doi]
PST - ppublish
SO  - Kidney Int. 2003 Jul;64(1):25-35. doi: 10.1046/j.1523-1755.2003.00067.x.