PMID- 12787678
OWN - NLM
STAT- MEDLINE
DCOM- 20030724
LR  - 20190710
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 329
IP  - 4
DP  - 2003 Jun 13
TI  - The sensitivity of lipid domains to small perturbations demonstrated by the 
      effect of Triton.
PG  - 793-9
AB  - The hypothesis of lipid rafts describes functional domains in biological 
      membranes. It is often assumed that rafts form by spontaneous de-mixing of 
      certain lipids and that they can be isolated as detergent-resistant membrane 
      particles (DRMs) using the detergent Triton X-100 (TX). Here, we present a model 
      that describes the process of domain formation in membranes in the presence and 
      in the absence of TX. We measure the interactions between TX and an equimolar 
      mixture of sphingomyelin (SM), cholesterol (Cho), and 
      1-palmitoyl-2-oleoyl-3-sn-glycero-phosphatidylcholine (POPC) (1:1:1, mol) by 
      means of isothermal titration calorimetry. Comparison with pure POPC membranes 
      reveals a very unfavorable interaction between TX and SM/Cho, which causes a 
      substantial tendency to segregate these molecules into separate, DRM-like 
      (SM-rich) and fluid (TX-rich), domains. If rafts are indeed formed by spontaneous 
      de-mixing of PC and SM/Cho, they must be very sensitive, and perturbations caused 
      by techniques used to study rafts could lead to misleading results. If, however, 
      rafts are much more stable than PC-SM-Cho domains, there must be an unknown raft 
      stabilizer. Subtle changes of such a promoter could serve to modulate raft 
      function.
FAU - Heerklotz, Heiko
AU  - Heerklotz H
AD  - Biozentrum of the University of Basel, Division of Biophysical Chemistry, 
      Klingelbergstr. 70, CH-4056, Basel, Switzerland. heiko.heerklotz@unibas.ch
FAU - Szadkowska, Halina
AU  - Szadkowska H
FAU - Anderson, Thomas
AU  - Anderson T
FAU - Seelig, Joachim
AU  - Seelig J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Detergents)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Sphingomyelins)
RN  - 9002-93-1 (Octoxynol)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
SB  - IM
MH  - Animals
MH  - Calorimetry, Differential Scanning
MH  - Cholesterol/*chemistry
MH  - Detergents/*pharmacology
MH  - Humans
MH  - Lipid Bilayers/*metabolism
MH  - Membrane Microdomains/*chemistry/drug effects
MH  - Octoxynol/*pharmacology
MH  - Phosphatidylcholines/*chemistry
MH  - Sensitivity and Specificity
MH  - Sphingomyelins/*chemistry
EDAT- 2003/06/06 05:00
MHDA- 2003/07/25 05:00
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2003/07/25 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
AID - S0022283603005047 [pii]
AID - 10.1016/s0022-2836(03)00504-7 [doi]
PST - ppublish
SO  - J Mol Biol. 2003 Jun 13;329(4):793-9. doi: 10.1016/s0022-2836(03)00504-7.