PMID- 12788506
OWN - NLM
STAT- MEDLINE
DCOM- 20030814
LR  - 20191210
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 126
IP  - 1
DP  - 2003 Jun 15
TI  - A model experimental system for monitoring changes in sensory neuron phenotype 
      evoked by tooth injury.
PG  - 99-109
AB  - The dental pulp is a favorable model for studies of interactions between 
      nociceptive sensory neurons and their peripheral target tissues. In the present 
      study, we retrogradely labeled pulpal afferent neurons with an improved method 
      that permits monitoring of changes in neuronal phenotype in response to 
      controlled tooth injuries. The capacity of retrograde neuronal tracers to diffuse 
      through dentinal tubules was exploited, thereby avoiding the severe injury to the 
      pulp associated with previous tracer application methods. The strategy was to 
      apply the durable fluorescent tracer, Fluoro-gold (FG), to exposed dentin in the 
      floor of shallow cavities in molars, in order to pre-label pulpal neurons in 
      trigeminal ganglia of young adult Sprague-Dawley rats. A high percentage of pupal 
      afferent neurons were retrogradely labeled by application of FG to exposed dentin 
      and the FG fluorescent signal persisted in most labeled neurons for at least 8 
      weeks. Following tracer application to dentin, the pulp tissue appeared normal 
      histologically, with the exception that a layer of reactive dentin was deposited 
      at the pulp-dentin border beneath the shallow cavities. Assessment of expression 
      of calcitonin gene-related peptide (CGRP) and brain derived neurotrophic factor 
      (BDNF) indicated that pulpal neurons remained in a quiescent, baseline condition 
      cytochemically following application of tracer to cavities in dentin and 
      upregulation of these markers could be detected in neurons that projected to 
      teeth that received a test injury subsequent to tracer application. Thus, 
      labeling of trigeminal neurons via dentinal tubules provides the basis for a 
      useful model for precisely assessing properties of pulpal afferents in both 
      quiescent and activated states.
FAU - Pan, Yan
AU  - Pan Y
AD  - Department of Anatomy, University of Mississippi Medical Center, 2500 North State 
      Street, Jackson, MS 29216, USA.
FAU - Wheeler, Esther F
AU  - Wheeler EF
FAU - Bernanke, Jayne M
AU  - Bernanke JM
FAU - Yang, Hong
AU  - Yang H
FAU - Naftel, John P
AU  - Naftel JP
LA  - eng
GR  - R01 DE09137/DE/NIDCR NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Stilbamidines)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/genetics
MH  - Calcitonin Gene-Related Peptide/biosynthesis/genetics
MH  - Dental Pulp/injuries/*innervation/*metabolism
MH  - Extravasation of Diagnostic and Therapeutic Materials
MH  - Female
MH  - Fluorescent Dyes/administration & dosage/pharmacokinetics
MH  - Molar/injuries/innervation/metabolism
MH  - Neurons, Afferent/metabolism/*pathology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Staining and Labeling/methods
MH  - *Stilbamidines
MH  - Tooth Injuries/*metabolism/physiopathology
MH  - Trigeminal Ganglion/metabolism/pathology/physiopathology
EDAT- 2003/06/06 05:00
MHDA- 2003/08/15 05:00
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2003/08/15 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
AID - S0165027003000712 [pii]
AID - 10.1016/s0165-0270(03)00071-2 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2003 Jun 15;126(1):99-109. doi: 
      10.1016/s0165-0270(03)00071-2.