PMID- 12788639
OWN - NLM
STAT- MEDLINE
DCOM- 20030619
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 310
IP  - 1
DP  - 2003 May 25
TI  - The mature reverse transcriptase molecules in virions of mouse mammary tumor 
      virus possess protease-derived sequences.
PG  - 157-62
AB  - Our efforts to express in bacteria the enzymatically active reverse transcriptase 
      (RT) of mouse mammary tumor virus (MMTV) have shown that the RT is active only 
      after adding 27 amino acid residues, which are derived from the end of the pro 
      gene, to the amino-terminus of the RT (Biochem, J. (1998) 329, 579-587). In the 
      present study we have tested whether the mature RT found in virions is also fused 
      to protease-derived sequences. To this end, we have analyzed the RT molecules in 
      virions of MMTV by using two antisera directed against peptides, derived from 
      either the carboxyl-terminus of MMTV protease or the middle of MMTV RT. The data 
      suggest that the mature RT, located in virions, contains at its amino-terminus 
      sequences from the carboxyl-terminus of the protease protein. This finding 
      supports previous suggestions that MMTV RT is a transframe protein (derived from 
      both pro and pol reading frames of MMTV) and that amino acid residues located at 
      the carboxyl-terminus of the protease have a dual usage as integral parts of both 
      the protease and the RT enzymes.
FAU - Entin-Meer, Michal
AU  - Entin-Meer M
AD  - Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv 
      University, Tel Aviv 69978, Israel.
FAU - Avidan, Orna
AU  - Avidan O
FAU - Hizi, Amnon
AU  - Hizi A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - EC 3.4.- (Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Endopeptidases/*chemistry
MH  - Mammary Tumor Virus, Mouse/*enzymology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Molecular Sequence Data
MH  - RNA-Directed DNA Polymerase/*chemistry
MH  - Rabbits
MH  - Virion/*enzymology
EDAT- 2003/06/06 05:00
MHDA- 2003/06/20 05:00
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2003/06/20 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
AID - S0042682203001132 [pii]
AID - 10.1016/s0042-6822(03)00113-2 [doi]
PST - ppublish
SO  - Virology. 2003 May 25;310(1):157-62. doi: 10.1016/s0042-6822(03)00113-2.