PMID- 12788771
OWN - NLM
STAT- MEDLINE
DCOM- 20031001
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 69
IP  - 6
DP  - 2003 Jun
TI  - Characterization of an autotrophic nitrogen-removing biofilm from a highly loaded 
      lab-scale rotating biological contactor.
PG  - 3626-35
AB  - In this study, a lab-scale rotating biological contactor (RBC) treating a 
      synthetic NH(4)(+) wastewater devoid of organic carbon and showing high N losses 
      was examined for several important physiological and microbial characteristics. 
      The RBC biofilm removed 89% +/- 5% of the influent N at the highest surface load 
      of approximately 8.3 g of N m(-2) day(-1), with N(2) as the main end product. In 
      batch tests, the RBC biomass showed good aerobic and anoxic ammonium oxidation 
      (147.8 +/- 7.6 and 76.5 +/- 6.4 mg of NH(4)(+)-N g of volatile suspended solids 
      [VSS](-1) day(-1), respectively) and almost no nitrite oxidation (< 1 mg of N g 
      of VSS(-1) day(-1)). The diversity of aerobic ammonia-oxidizing bacteria (AAOB) 
      and planctomycetes in the biofilm was characterized by cloning and sequencing of 
      PCR-amplified partial 16S rRNA genes. Phylogenetic analysis of the clones 
      revealed that the AAOB community was fairly homogeneous and was dominated by 
      Nitrosomonas-like species. Close relatives of the known anaerobic 
      ammonia-oxidizing bacterium (AnAOB) Kuenenia stuttgartiensis dominated the 
      planctomycete community and were most probably responsible for anoxic ammonium 
      oxidation in the RBC. Use of a less specific planctomycete primer set, not 
      amplifying the AnAOB, showed a high diversity among other planctomycetes, with 
      representatives of all known groups present in the biofilm. The spatial 
      organization of the biofilm was characterized using fluorescence in situ 
      hybridization (FISH) with confocal scanning laser microscopy (CSLM). The latter 
      showed that AAOB occurred side by side with putative AnAOB (cells hybridizing 
      with probe PLA46 and AMX820/KST1275) throughout the biofilm, while other 
      planctomycetes hybridizing with probe PLA886 (not detecting the known AnAOB) were 
      present as very conspicuous spherical structures. This study reveals that 
      long-term operation of a lab-scale RBC on a synthetic NH(4)(+) wastewater devoid 
      of organic carbon yields a stable biofilm in which two bacterial groups, thought 
      to be jointly responsible for the high autotrophic N removal, occur side by side 
      throughout the biofilm.
FAU - Pynaert, Kris
AU  - Pynaert K
AD  - Laboratory of Microbial Ecology and Technology, Faculty of Agricultural and 
      Applied Biological Sciences, Ghent University, B-9000 Ghent, Belgium.
FAU - Smets, Barth F
AU  - Smets BF
FAU - Wyffels, Stijn
AU  - Wyffels S
FAU - Beheydt, Daan
AU  - Beheydt D
FAU - Siciliano, Steven D
AU  - Siciliano SD
FAU - Verstraete, Willy
AU  - Verstraete W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*classification/genetics/metabolism
MH  - Biofilms/*classification/growth & development
MH  - *Bioreactors
MH  - DNA, Ribosomal/analysis
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Nitrogen/*metabolism
MH  - Nitrosomonas/classification/genetics/metabolism
MH  - Oxidation-Reduction
MH  - Polymerase Chain Reaction
MH  - Quaternary Ammonium Compounds/metabolism
MH  - RNA, Ribosomal, 16S
MH  - Waste Disposal, Fluid/*methods
PMC - PMC161519
EDAT- 2003/06/06 05:00
MHDA- 2003/10/02 05:00
PMCR- 2003/06/01
CRDT- 2003/06/06 05:00
PHST- 2003/06/06 05:00 [pubmed]
PHST- 2003/10/02 05:00 [medline]
PHST- 2003/06/06 05:00 [entrez]
PHST- 2003/06/01 00:00 [pmc-release]
AID - 2008 [pii]
AID - 10.1128/AEM.69.6.3626-3635.2003 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2003 Jun;69(6):3626-35. doi: 
      10.1128/AEM.69.6.3626-3635.2003.