PMID- 12791235
OWN - NLM
STAT- MEDLINE
DCOM- 20030917
LR  - 20061115
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 34
IP  - 3
DP  - 2003 May-Jun
TI  - Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory 
      syndrome virus and associations with the production of apoptogenic cytokines.
PG  - 249-60
AB  - Apoptosis was studied in the lungs of pigs during an infection with a European 
      strain of porcine reproductive and respiratory syndrome virus (PRRSV) and it was 
      examined if cytokines were involved in the induction of apoptosis. Twenty-two 4- 
      to 5-week-old gnotobiotic pigs were inoculated intranasally with 10(6.0) TCID50 
      of the Lelystad virus and euthanised between 1 and 52 days post inoculation (PI). 
      The lungs and broncho-alveolar lavage (BAL) cells were assessed both for virus 
      replication and apoptosis; BAL fluids were examined for interleukin (IL)-1, 
      tumour necrosis factor-alpha and IL-10. Double-labellings were conducted to 
      determine the relation between virus replication and apoptosis and to identify 
      the apoptotic cells. Apoptosis occurred in both infected and non-infected cells. 
      The percentages of infected cells, which were apoptotic, ranged between 9 and 39% 
      in the lungs and between 13 and 30% in the BAL cells. The majority of apoptotic 
      cells were non-infected. Non-infected apoptotic cells in the lungs were 
      predominantly monocytes/macrophages, whereas those in the broncho-alveolar spaces 
      were predominantly lymphocytes. The peak of apoptosis in the lungs at 14 days PI 
      was preceded by a peak of IL-1 and IL-10 production at 9 days PI, suggesting a 
      possible role of these cytokines in the induction of apoptosis in non-infected 
      interstitial monocytes/macrophages. However, the latter hypothesis was not 
      confirmed in vitro, since blood monocytes or alveolar macrophages did not undergo 
      apoptosis after treatment with recombinant porcine IL-1 or IL-10.
FAU - Labarque, Geoffrey
AU  - Labarque G
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, 9820 Merelbeke, Belgium.
FAU - Van Gucht, Steven
AU  - Van Gucht S
FAU - Nauwynck, Hans
AU  - Nauwynck H
FAU - Van Reeth, Kristien
AU  - Van Reeth K
FAU - Pensaert, Maurice
AU  - Pensaert M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Interleukin-1/metabolism/pharmacology
MH  - Interleukin-10/metabolism/pharmacology
MH  - Lung/*pathology/*virology
MH  - Macrophages, Alveolar/drug effects
MH  - Monocytes/drug effects
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine/virology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Virus Replication
EDAT- 2003/06/07 05:00
MHDA- 2003/09/18 05:00
CRDT- 2003/06/07 05:00
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2003/09/18 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
AID - 10.1051/vetres:2003001 [doi]
PST - ppublish
SO  - Vet Res. 2003 May-Jun;34(3):249-60. doi: 10.1051/vetres:2003001.