PMID- 12791648
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 102
IP  - 10
DP  - 2003 Nov 15
TI  - A dendritic cell vaccine against invasive aspergillosis in allogeneic 
      hematopoietic transplantation.
PG  - 3807-14
AB  - Dendritic cells (DCs) have a remarkable functional plasticity in response to 
      conidia and hyphae of the fungus Aspergillus fumigatus. In the present study we 
      sought to assess the capacity of DCs activated by live fungi or fungal RNA to 
      generate antifungal immunity in vivo. We found that both human and murine DCs 
      pulsed with live fungi or transfected with fungal RNA underwent functional 
      maturation, as revealed by the up-regulated expression of histocompatibility 
      class II antigen and costimulatory molecules and the production of interleukin 12 
      (IL-12) in response to conidia or conidial RNA and of IL-4/IL-10 in response to 
      hyphae or hyphal RNA. DCs pulsed with conidia or transfected with conidial RNA 
      activated antigen-specific, interferon gamma (IFN-gamma)-producing T lymphocytes 
      in vitro and in vivo on adoptive transfer in mice otherwise susceptible to 
      aspergillosis. TH1-dependent antifungal resistance could also be induced in mice 
      receiving allogeneic bone marrow transplants and was associated with an 
      accelerated recovery of myeloid and lymphoid cells. Because the efficacy of the 
      infusion of DCs was superior to that obtained on the adoptive transfer of 
      Aspergillus-specific T cells, these results indicate the vaccinating potential of 
      DCs pulsed with Aspergillus conidia or conidial RNA in hematopoietic 
      transplantation.
FAU - Bozza, Silvia
AU  - Bozza S
AD  - Microbiology Section, Department of Experimental Medicine and Biochemical 
      Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
FAU - Perruccio, Katia
AU  - Perruccio K
FAU - Montagnoli, Claudia
AU  - Montagnoli C
FAU - Gaziano, Roberta
AU  - Gaziano R
FAU - Bellocchio, Silvia
AU  - Bellocchio S
FAU - Burchielli, Emanuela
AU  - Burchielli E
FAU - Nkwanyuo, Gabriel
AU  - Nkwanyuo G
FAU - Pitzurra, Lucia
AU  - Pitzurra L
FAU - Velardi, Andrea
AU  - Velardi A
FAU - Romani, Luigina
AU  - Romani L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030605
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukins)
RN  - 0 (RNA, Fungal)
RN  - 0 (Vaccines)
SB  - IM
MH  - Adoptive Transfer/*methods
MH  - Animals
MH  - Aspergillosis/*therapy
MH  - Aspergillus/genetics/immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Histocompatibility Antigens Class II/biosynthesis
MH  - Humans
MH  - Interleukins/biosynthesis
MH  - Mice
MH  - Mice, Inbred Strains
MH  - RNA, Fungal
MH  - Th1 Cells/immunology
MH  - Transfection
MH  - Transplantation, Homologous
MH  - Up-Regulation
MH  - Vaccines
EDAT- 2003/06/07 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/06/07 05:00
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
AID - S0006-4971(20)50388-1 [pii]
AID - 10.1182/blood-2003-03-0748 [doi]
PST - ppublish
SO  - Blood. 2003 Nov 15;102(10):3807-14. doi: 10.1182/blood-2003-03-0748. Epub 2003 
      Jun 5.