PMID- 12791652
OWN - NLM
STAT- MEDLINE
DCOM- 20031017
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 102
IP  - 6
DP  - 2003 Sep 15
TI  - Most lymphoid organ dendritic cell types are phenotypically and functionally 
      immature.
PG  - 2187-94
AB  - Dendritic cells (DCs) have been thought to follow a life history, typified by 
      Langerhans cells (LCs), with 2 major developmental stages: an immature stage that 
      captures antigens in the periphery and a mature stage that presents those 
      antigens in the lymphoid organs. However, a systematic assessment of the maturity 
      of lymphoid organ DCs has been lacking. We have analyzed the maturity of the DC 
      types found in the steady state in the spleen, lymph nodes (LNs), and thymus. The 
      DCs that migrate into the iliac, mesenteric, mediastinal, or subcutaneous LNs 
      from peripheral tissues were mature and therefore could not process and present 
      newly encountered antigens. However, all the other DC types were phenotypically 
      and functionally immature: they expressed low levels of surface major 
      histocompatibility complex class II (MHC II) and CD86, accumulated MHC II in 
      their endosomes, and could present newly encountered antigens. These immature DCs 
      could be induced to mature by culture in vitro or by inoculation of inflammatory 
      stimuli in vivo. Therefore, the lymphoid organs contain a large cohort of 
      immature DCs, most likely for the maintenance of peripheral tolerance, which can 
      respond to infections reaching those organs and mature in situ.
FAU - Wilson, Nicholas S
AU  - Wilson NS
AD  - Immunology Division, The Walter and Eliza Hall Institute of Medical Research, 1G 
      Royal Parade, Melbourne, Victoria 3050, Australia.
FAU - El-Sukkari, Dima
AU  - El-Sukkari D
FAU - Belz, Gabrielle T
AU  - Belz GT
FAU - Smith, Christopher M
AU  - Smith CM
FAU - Steptoe, Raymond J
AU  - Steptoe RJ
FAU - Heath, William R
AU  - Heath WR
FAU - Shortman, Ken
AU  - Shortman K
FAU - Villadangos, Jose A
AU  - Villadangos JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030605
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/immunology
MH  - Cell Division/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Histocompatibility Antigens Class II/analysis
MH  - Immune Tolerance/*immunology
MH  - Immunophenotyping
MH  - In Vitro Techniques
MH  - Lymph Nodes/chemistry/cytology/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Spleen/chemistry/cytology/immunology
MH  - Thymus Gland/chemistry/cytology/immunology
MH  - Tissue Fixation
EDAT- 2003/06/07 05:00
MHDA- 2003/10/18 05:00
CRDT- 2003/06/07 05:00
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2003/10/18 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
AID - S0006-4971(20)50512-0 [pii]
AID - 10.1182/blood-2003-02-0513 [doi]
PST - ppublish
SO  - Blood. 2003 Sep 15;102(6):2187-94. doi: 10.1182/blood-2003-02-0513. Epub 2003 Jun 
      5.