PMID- 12791819
OWN - NLM
STAT- MEDLINE
DCOM- 20030724
LR  - 20191210
IS  - 0161-5505 (Print)
IS  - 0161-5505 (Linking)
VI  - 44
IP  - 6
DP  - 2003 Jun
TI  - Calf flow reserve with H(2)(15)O PET as a quantifiable index of lower extremity 
      flow.
PG  - 915-9
AB  - Objective measures of recruitable blood flow are of importance in angiogenesis 
      trials. We validated a new PET-derived flow reserve (FR) measurement in healthy 
      subjects and subjects with peripheral artery disease (PAD). METHODS: Five healthy 
      volunteers and 5 subjects with PAD underwent cannulation of the femoral artery 
      and vein. Basal and maximal flow (100 micro g/kg/min of adenosine infused 
      intraarterially) in the lower extremity was determined using thermodilution (TD) 
      techniques. Subjects then underwent plethysmography (PL) followed by PET 
      measurements of blood flow at the calf level. For the PET studies, a transmission 
      scan followed by injection of 1.85 GBq (50 mCi) H(2)(15)O and dynamic scanning 
      for 5 min were acquired in five 1-min frames. Regions of interest were drawn on 
      successive PET image slices, and radioactivity was quantified from the 
      first-minute scan after injection. FR for each of the 3 modalities was expressed 
      as the ratio of adenosine to basal flow. RESULTS: PET-derived FR correlated 
      strongly with TD (r = 0.82; P = 0.004) but not with PL (r = 0.17; P = 0.85). The 
      mean average difference in FR between healthy volunteers and PAD subjects was 
      13.0 with PET and 4.5 with TD. The intra- and intersubject variability for PET 
      expressed as the coefficient of variation was 10.5% and 29.0% for healthy 
      subjects and 7.0% and 52.9% in PAD, respectively. CONCLUSION: As expected, FR was 
      significantly lower in PAD subjects compared with healthy subjects as assessed 
      with TD and PET but not with PL. PET-derived FR appears to be reproducible and 
      generates sharper and higher indices of recruitable flow in healthy subjects and 
      PAD. These findings have implications for the use of PET-derived FR as a 
      sensitive index of recruitable flow in angiogenesis trials.
FAU - Schmidt, Michael A
AU  - Schmidt MA
AD  - Section of Vascular Medicine, Division of Cardiology, University of Michigan, Ann 
      Arbor, Michigan, USA.
FAU - Chakrabarti, Anjan
AU  - Chakrabarti A
FAU - Shamim-Uzzaman, Qurratul
AU  - Shamim-Uzzaman Q
FAU - Kaciroti, Niko
AU  - Kaciroti N
FAU - Koeppe, Robert A
AU  - Koeppe RA
FAU - Rajagopalan, Sanjay
AU  - Rajagopalan S
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - J Nucl Med
JT  - Journal of nuclear medicine : official publication, Society of Nuclear Medicine
JID - 0217410
RN  - 0 (Oxygen Radioisotopes)
RN  - 0 (Radiopharmaceuticals)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine
MH  - Adult
MH  - Arteries/diagnostic imaging/physiopathology
MH  - Blood Flow Velocity
MH  - Female
MH  - Femoral Artery/physiopathology
MH  - Humans
MH  - *Hydrogen/pharmacokinetics
MH  - Lower Extremity/blood supply/diagnostic imaging
MH  - Male
MH  - Middle Aged
MH  - Muscle, Skeletal/*blood supply/*diagnostic imaging
MH  - Neovascularization, Physiologic
MH  - *Oxygen Radioisotopes/pharmacokinetics
MH  - Peripheral Vascular Diseases/*diagnostic imaging/physiopathology
MH  - Plethysmography
MH  - Radiopharmaceuticals/pharmacokinetics
MH  - Regional Blood Flow
MH  - Thermodilution
MH  - Thigh/blood supply/diagnostic imaging
MH  - Tomography, Emission-Computed/methods
EDAT- 2003/06/07 05:00
MHDA- 2003/07/25 05:00
CRDT- 2003/06/07 05:00
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2003/07/25 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
PST - ppublish
SO  - J Nucl Med. 2003 Jun;44(6):915-9.