PMID- 12792774
OWN - NLM
STAT- MEDLINE
DCOM- 20040318
LR  - 20061115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 23
IP  - 1
DP  - 2003 Jul
TI  - Numerical aberrations of chromosome 17 and the 9p21 locus are independent 
      predictors of tumor recurrence in non-invasive transitional cell carcinoma of the 
      urinary bladder.
PG  - 41-8
AB  - Since the majority of patients with non-invasive (stage pTa) bladder carcinoma 
      experience tumor recurrence, the identification of prognostic markers for 
      assessing the biologic behavior of tumors is of high clinical interest. In this 
      retrospective study, we investigated the prognostic value of numerical 
      aberrations of chromosomes 3, 7, 17 and of the 9p21 gene locus in 71 pTa bladder 
      carcinomas using the UroVysion test. This recently developed multi-probe 
      fluorescence in situ hybridization (FISH) test, which simultaneously stains the 
      9p21 locus and the centromer regions of chromosomes 3, 7 and 17, was applied on 
      paraffin sections from formalin-fixed tumor specimens. All tumors were evaluated 
      for twelve different criteria, among which were: polysomy 3, 7 or 17 in > or =10% 
      of tumor cells, pentasomy or higher polysomy 3, 7 or 17 in > or =1 tumor cell, 
      and loss of both 9p21 alleles in > or =10% of tumor cells. The latter parameter 
      was the most frequent chromosomal aberration (detected in 83% of the tumors), 
      while polysomies 3, 7 and 17 were registered in 27, 13 and 12% of the cases, 
      respectively. Most of the parameters were found at higher frequencies in G3 
      tumors compared to G1 or G2 tumors. None of the parameters correlated with 
      progression-free survival, but polysomy 3 (p=0.029), polysomy of at least one of 
      the chromosomes 3, 7 or 17 (p=0.032), pentasomy/higher polysomy 17 (p=0.007), and 
      loss of 9p21 (p=0.026), correlated significantly with recurrence-free survival. 
      Of these, pentasomy/higher polysomy 17 (p=0.015) and loss of 9p21 (p=0.036) were 
      identified as independent predictors of tumor recurrence in a multivariate Cox 
      regression analysis that also included tumor grade. In conclusion, we suggest 
      that evaluation of numerical aberrations of chromosome 17 and the 9p21 locus may 
      represent a useful tool to assess tumor recurrence of pTa bladder carcinomas more 
      accurately.
FAU - Kruger, Stefan
AU  - Kruger S
AD  - Institute of Pathology, University of Lubeck, Ratzeburger Allee 160, D-23538 
      Lubeck, Germany. krueger@patho.mu-luebeck.de
FAU - Mess, Franziska
AU  - Mess F
FAU - Bohle, Andreas
AU  - Bohle A
FAU - Feller, Alfred C
AU  - Feller AC
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17/*ultrastructure
MH  - Chromosomes, Human, Pair 9/*ultrastructure
MH  - Disease Progression
MH  - Disease-Free Survival
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - Recurrence
MH  - Time Factors
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 2003/06/07 05:00
MHDA- 2004/03/19 05:00
CRDT- 2003/06/07 05:00
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2004/03/19 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Jul;23(1):41-8.