PMID- 12792872
OWN - NLM
STAT- MEDLINE
DCOM- 20030724
LR  - 20220227
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 187
IP  - 12
DP  - 2003 Jun 15
TI  - Evidence of viral replication in circulating dendritic cells during hepatitis C 
      virus infection.
PG  - 1951-8
AB  - The existence of extrahepatic sites of hepatitis C virus (HCV) replication has 
      been proposed as a mechanism responsible for the poor antiviral immune response 
      found in chronic infection. Dendritic cells (DCs), as unique antigen-presenting 
      cells able to induce a primary immune response, are prime targets of persistent 
      viruses. From 24 blood samples obtained from HCV-seropositive patients, 
      peripheral blood DCs (PBDCs) were purified. HCV genomic sequences were 
      specifically detected by reverse-transcription polymerase chain reaction in 6 of 
      24 PBDC pellets, and replicative-strand RNA also was found in 3 of 24 cell 
      purifications. Analysis of the HCV quasi-species distribution in the PBDC 
      population of 1 patient showed the presence of a dominant variant different from 
      that found in plasma with respect to the primary amino-acid sequence and 
      physicochemical profile of the hypervariable region 1 of glycoprotein E2. These 
      data strongly suggest that PBDCs constitute a reservoir in which HCV replication 
      takes place during natural infection.
FAU - Goutagny, Nadege
AU  - Goutagny N
AD  - Unite Mixte de Recherche 2142, Centre National de la Recherche Scientifique 
      Biomerieux, Lyon, France.
FAU - Fatmi, Ahmed
AU  - Fatmi A
FAU - De Ledinghen, Victor
AU  - De Ledinghen V
FAU - Penin, Francois
AU  - Penin F
FAU - Couzigou, Patrice
AU  - Couzigou P
FAU - Inchauspe, Genevieve
AU  - Inchauspe G
FAU - Bain, Christine
AU  - Bain C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030529
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Dendritic Cells/*virology
MH  - Female
MH  - Genes, Viral
MH  - Genome, Viral
MH  - Hepacivirus/chemistry/genetics/isolation & purification/*physiology
MH  - Hepatitis C, Chronic/*virology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Species Specificity
MH  - *Virus Replication
EDAT- 2003/06/07 05:00
MHDA- 2003/07/25 05:00
CRDT- 2003/06/07 05:00
PHST- 2002/10/15 00:00 [received]
PHST- 2003/01/22 00:00 [accepted]
PHST- 2003/06/07 05:00 [pubmed]
PHST- 2003/07/25 05:00 [medline]
PHST- 2003/06/07 05:00 [entrez]
AID - JID30200 [pii]
AID - 10.1086/375350 [doi]
PST - ppublish
SO  - J Infect Dis. 2003 Jun 15;187(12):1951-8. doi: 10.1086/375350. Epub 2003 May 29.