PMID- 1279518
OWN - NLM
STAT- MEDLINE
DCOM- 19921215
LR  - 20190726
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 422
IP  - 1
DP  - 1992 Oct
TI  - Effect of thapsigargin and caffeine on Ca2+ homeostasis in HeLa cells: 
      implications for histamine-induced Ca2+ oscillations.
PG  - 40-7
AB  - Several studies have already established that the stimulation of H1 receptors by 
      exogenous histamine induces intracellular Ca2+ oscillations in HeLa cells. The 
      molecular mechanism underlying this oscillatory process remains, however, 
      unclear. A series of fura-2 experiments was undertaken in which the nature of the 
      Ca2+ pools involved in the histamine-induced Ca2+ oscillations was investigated 
      using the tumour promoter agent thapsigargin (TG) and the Ca(2+)-induced 
      Ca(2+)-release promoter, caffeine. The results obtained indicate first that TG 
      causes a gradual increase in cytosolic Ca2+ without inducing internal Ca2+ 
      oscillations, and second that TG and histamine share common internal Ca2+ storage 
      sites. The latter conclusion was derived from experiments performed in the 
      absence of external Ca2+, where the addition of TG before histamine resulted in a 
      total inhibition of the Ca2+ response linked to H1 receptor stimulation, whereas 
      the addition of histamine before TG decreased by more than 90% the TG-induced 
      Ca2+ release. Finally; TG was found to inhibit irreversibly histamine-induced 
      Ca2+ oscillations when added to the bathing medium during the oscillatory 
      process. The effect of caffeine at concentrations ranging from 1 mM to 10 mM on 
      intracellular Ca2+ homeostasis was also investigated. The results obtained show 
      that caffeine does not affect systematically the internal Ca2+ concentration in 
      resting and TG-stimulated HeLa cells, but increases the Ca2+ sequestration 
      ability of inositol-trisphosphate (InsP3)-related Ca2+ stores.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Diarra, A
AU  - Diarra A
AD  - Departement de physiologie, Universite de Montreal, Quebec, Canada.
FAU - Sauve, R
AU  - Sauve R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Terpenes)
RN  - 3G6A5W338E (Caffeine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 820484N8I3 (Histamine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Caffeine/*pharmacology
MH  - Calcium/*metabolism
MH  - HeLa Cells/*metabolism
MH  - Histamine/*pharmacology
MH  - Histamine Release/drug effects
MH  - Homeostasis
MH  - Humans
MH  - Inositol 1,4,5-Trisphosphate/pharmacology
MH  - Oscillometry
MH  - Terpenes/*pharmacology
MH  - Thapsigargin
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 10.1007/BF00381511 [doi]
PST - ppublish
SO  - Pflugers Arch. 1992 Oct;422(1):40-7. doi: 10.1007/BF00381511.