PMID- 12796819
OWN - NLM
STAT- MEDLINE
DCOM- 20050401
LR  - 20131121
IS  - 0582-9879 (Print)
IS  - 0582-9879 (Linking)
VI  - 35
IP  - 6
DP  - 2003 Jun
TI  - Dynamic investigation of leukocyte-endothelial cell adhesion interaction under 
      fluid shear stress in vitro.
PG  - 567-72
AB  - To establish a method to investigate the dynamic adhesion between leukocytes and 
      human umbilical vein endothelial cells (HUVECs) under definite shear stress. A 
      parallel plate flow chamber system was developed to produce the definite shear 
      stress in vitro. After the cultured HUVECs were loaded in the flow chamber, the 
      circulation solution containing acridine orange (AO)-labeled leukocytes was 
      perfused to flow through chamber at 0.71 dynes/cm(2). In this case, 
      leukocyte-endothelial cell adhesion process was induced. Lipopolysaccharide(LPS) 
      was used as the chemical stimulus and dexamethasone(DXM) was used as the 
      anti-inflammatory reagent. The adhesion process was recorded in videotape by 
      Olympus IX70 fluorescence microscope and CCD-camera. Then the number of adhesion 
      leukocyte, slow and fast rolling velocities of leukocytes on the surface of 
      HUVECs were measured based on the captured images. The number of static adhering 
      and slow rolling leukocytes on the HUVECs treated with LPS was significantly 
      increased by 23.7-fold and 4.1-fold compared with that of the control group. 
      Meanwhile, both the slow and fast rolling velocities of the leukocytes on HUVECs 
      treated with LPS were significantly decreased by 25.6% and 26.1%. When HUVECs 
      were treated with both LPS and DXM, the effect of LPS was inhibited obviously. 
      This developed method can be used in studying ECs adhesion function affected by 
      different chemical and physical stimulus and evaluating the various compounds 
      interfering with cell adhesion.
FAU - Ling, Xu
AU  - Ling X
AD  - Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, 
      China.
FAU - Ye, Jian-Feng
AU  - Ye JF
FAU - Zheng, Xiao-Xiang
AU  - Zheng XX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
JT  - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
JID - 20730160R
RN  - 0 (Lipopolysaccharides)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Endothelial Cells/drug effects/*physiology
MH  - Female
MH  - Humans
MH  - Leukocytes/drug effects/*physiology
MH  - Lipopolysaccharides/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rheology
MH  - Shear Strength
MH  - Stress, Mechanical
MH  - Umbilical Veins/cytology
EDAT- 2003/06/11 05:00
MHDA- 2005/04/02 09:00
CRDT- 2003/06/11 05:00
PHST- 2003/06/11 05:00 [pubmed]
PHST- 2005/04/02 09:00 [medline]
PHST- 2003/06/11 05:00 [entrez]
PST - ppublish
SO  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Jun;35(6):567-72.