PMID- 12797524
OWN - NLM
STAT- MEDLINE
DCOM- 20031010
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 49
IP  - 3
DP  - 2003 Mar
TI  - Necrotic feature of the trophoblasts lacking HLA-G expression in normal and 
      pre-eclamptic placentas.
PG  - 174-82
AB  - PROBLEM: Human leukocyte antigen (HLA)-G is thought to be expressed in all 
      placental extravillous trophoblasts (EXTs). In pre-eclamptic placentas, a lack of 
      HLA-G expression on EXTs had been found, and deduced as a possible cause of 
      pre-eclampsia. However, a subset of EXTs lacking expression of HLA-G can also be 
      found in normal placenta. Therefore, we sought to compare these cells in normal 
      and pre-eclamptic placentas. METHODS OF STUDY: Frozen sections of normal and 
      pre-eclamptic placentas were examined by immunohistochemical staining using HLA-G 
      monoclonal antibody 87G, histochemical enzymatic analysis of succinate 
      dehydrogenase (SDH) and ultrastructural analysis. RESULTS: A subset of EXTs 
      lacking HLA-G expression was found in both normal and pre-eclamptic placentas. 
      These cells showed necrotic features such as the swelling of cells, 
      eosin-achromatophilia, the loss of SDH activity and swelling mitochondria. Cells 
      from both tissues were identical with regard to these features. CONCLUSION: The 
      features of the EXTs lacking HLA-G expression indicated they had undergone 
      necrosis and thus could not express HLA-G protein. Therefore, an alternative 
      interpretation to the lack of HLA-G expression in pre-eclamptic placentas is that 
      it is the result of cell death and not the cause.
FAU - Sageshima, Noriko
AU  - Sageshima N
AD  - Department of Legal Medicine, Nara Medical University, Nara, Japan.
FAU - Ishitani, Akiko
AU  - Ishitani A
FAU - Omura, Motoko
AU  - Omura M
FAU - Akasaki, Masayoshi
AU  - Akasaki M
FAU - Umekage, Hideshi
AU  - Umekage H
FAU - Katabuchi, Hidetaka
AU  - Katabuchi H
FAU - Okamura, Hitoshi
AU  - Okamura H
FAU - Hatake, Katsuhiko
AU  - Hatake K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Female
MH  - HLA Antigens/*biosynthesis
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - K562 Cells
MH  - Microscopy, Electron
MH  - Necrosis
MH  - Placenta/immunology/*metabolism/pathology
MH  - Pre-Eclampsia/immunology/*metabolism/pathology
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Pregnancy Trimester, Second
MH  - Pregnancy Trimester, Third
MH  - Trophoblasts/*metabolism/pathology/ultrastructure
MH  - Tumor Cells, Cultured
EDAT- 2003/06/12 05:00
MHDA- 2003/10/11 05:00
CRDT- 2003/06/12 05:00
PHST- 2003/06/12 05:00 [pubmed]
PHST- 2003/10/11 05:00 [medline]
PHST- 2003/06/12 05:00 [entrez]
AID - 10.1034/j.1600-0897.2003.00010.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2003 Mar;49(3):174-82. doi: 
      10.1034/j.1600-0897.2003.00010.x.