PMID- 12798291
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20220310
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 258
IP  - 2
DP  - 2003 Jun 15
TI  - Nitric oxide acts in a positive feedback loop with BDNF to regulate neural 
      progenitor cell proliferation and differentiation in the mammalian brain.
PG  - 319-33
AB  - Nitric oxide (NO) is believed to act as an intercellular signal that regulates 
      synaptic plasticity in mature neurons. We now report that NO also regulates the 
      proliferation and differentiation of mouse brain neural progenitor cells (NPCs). 
      Treatment of dissociated mouse cortical neuroepithelial cluster cell cultures 
      with the NO synthase inhibitor L-NAME or the NO scavenger hemoglobin increased 
      cell proliferation and decreased differentiation of the NPCs into neurons, 
      whereas the NO donor sodium nitroprusside inhibited NPC proliferation and 
      increased neuronal differentiation. Brain-derived neurotrophic factor (BDNF) 
      reduced NPC proliferation and increased the expression of neuronal NO synthase 
      (nNOS) in differentiating neurons. The stimulatory effect of BDNF on neuronal 
      differentation of NPC was blocked by L-NAME and hemoglobin, suggesting that NO 
      produced by the latter cells inhibited proliferation and induced neuronal 
      differentiation of neighboring NPCs. A similar role for NO in regulating the 
      switch of neural stem cells from proliferation to differentiation in the adult 
      brain is suggested by data showing that NO synthase inhibition enhances NPC 
      proliferation and inhibits neuronal differentiation in the subventricular zone of 
      adult mice. These findings identify NO as a paracrine messenger stimulated by 
      neurotrophin signaling in newly generated neurons to control the proliferation 
      and differentiation of NPC, a novel mechanism for the regulation of developmental 
      and adult neurogenesis.
FAU - Cheng, Aiwu
AU  - Cheng A
AD  - Laboratory of Neurosciences, National Institute on Aging Gerontology Research 
      Center, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
FAU - Wang, Shuqin
AU  - Wang S
FAU - Cai, Jingli
AU  - Cai J
FAU - Rao, Mahendra S
AU  - Rao MS
FAU - Mattson, Mark P
AU  - Mattson MP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, mouse)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/*cytology/*embryology/growth & development/metabolism
MH  - Brain-Derived Neurotrophic Factor/pharmacology/*physiology
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Feedback
MH  - Female
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Neurons/cytology/metabolism
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase/antagonists & inhibitors/biosynthesis
MH  - Nitric Oxide Synthase Type I
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor, trkB/genetics/metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology/metabolism
EDAT- 2003/06/12 05:00
MHDA- 2003/07/12 05:00
CRDT- 2003/06/12 05:00
PHST- 2003/06/12 05:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2003/06/12 05:00 [entrez]
AID - S0012160603001209 [pii]
AID - 10.1016/s0012-1606(03)00120-9 [doi]
PST - ppublish
SO  - Dev Biol. 2003 Jun 15;258(2):319-33. doi: 10.1016/s0012-1606(03)00120-9.