PMID- 12798875
OWN - NLM
STAT- MEDLINE
DCOM- 20030703
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 79
IP  - 6
DP  - 2003 Jun
TI  - Chromosomal information derived from single blastomeres isolated from 
      cleavage-stage embryos and cultured in vitro.
PG  - 1304-11
AB  - OBJECTIVE: To evaluate the potential of proliferation of single blastomeres 
      isolated from human cleavage-stage embryos for use in preimplantation genetic 
      diagnosis of chromosomal abnormalities. DESIGN: A laboratory study of chromosomal 
      content of blastomeres isolated from embryos of patients from an in vitro 
      fertilization program. SETTING: University hospital laboratory. PATIENT(S): 
      Couples undergoing IVF or ICSI. INTERVENTION(S): Blastomeres were isolated from 
      normally fertilized cleavage-stage human embryos, cultured in vitro or fixed 
      immediately, and analyzed by fluorescence in situ hybridization (FISH) probes. 
      MAIN OUTCOME MEASURES: Chromosomal information yielded by blastomeres cultured in 
      vitro compared with those obtained from blastomeres that were processed for 
      chromosomal analysis directly after isolation. RESULT(S): The percentage of 
      cultured blastomeres that produced FISH results was significantly lower than the 
      percentage of blastomeres processed for FISH directly after isolation (72% vs. 
      90%). Lack of FISH results from cultured cells, which in most cases was related 
      to nuclear anomalies, was significantly more frequent among nondivided than 
      divided blastomeres (39% vs. 21%). Both cultured and noncultured cells showed 
      diploid, aneuploid and polyploid chromosome complements on FISH. Compared with 
      directly processed cells, cultured cells yielded a higher proportion of polyploid 
      patterns (22.9% vs. 6.1%). Of the cultured blastomeres that divided, 18% produced 
      progeny with mosaicism. CONCLUSION(S): Although blastomere culture may increase 
      the number of cells available for chromosomal analysis, the high frequency of 
      nuclear defects and the occurrence of polyploidy and mosaicism among cultured 
      cells discourage the use of blastomere isolation and proliferation strategy for 
      use in preimplantation genetic diagnosis.
FAU - Bielanska, Magdalena
AU  - Bielanska M
AD  - Department of Obstetrics and Gynecology, Royal Victoria Hospital, Montreal, 
      Quebec, Canada.
FAU - Tan, Seang Lin
AU  - Tan SL
FAU - Ao, Asangla
AU  - Ao A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - Blastomeres/*ultrastructure
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mosaicism
MH  - Polyploidy
MH  - Preimplantation Diagnosis
EDAT- 2003/06/12 05:00
MHDA- 2003/07/04 05:00
CRDT- 2003/06/12 05:00
PHST- 2003/06/12 05:00 [pubmed]
PHST- 2003/07/04 05:00 [medline]
PHST- 2003/06/12 05:00 [entrez]
AID - S001502820300356X [pii]
AID - 10.1016/s0015-0282(03)00356-x [doi]
PST - ppublish
SO  - Fertil Steril. 2003 Jun;79(6):1304-11. doi: 10.1016/s0015-0282(03)00356-x.