PMID- 12799295
OWN - NLM
STAT- MEDLINE
DCOM- 20030724
LR  - 20190616
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 996
DP  - 2003 May
TI  - Transplanted human cord blood cells give rise to hepatocytes in engrafted mice.
PG  - 174-85
AB  - Recent studies suggest that rodent hepatocytes may be derived from hematopoietic 
      stem cells. In the current study, the potential hematopoietic origin of 
      hepatocytes was addressed using xenogeneic transplantation of human cord blood 
      cells. CD34(+) or CD45(+) human cord blood cells were transplanted into 
      "conditioned" newborn NOD/SCID/beta2-microglobulin(null) mice. At 4 to 5 months 
      post-transplantation, livers of the recipient mice were cryosectioned and 
      examined for evidence of human hepatocyte engraftment using RT-PCR to detect 
      human albumin mRNA, immunohistochemistry to detect human hepatocytic proteins, 
      and fluorescence in situ hybridization (FISH) to detect the presence of human 
      centromeric DNA. Analysis of the bone marrow of transplanted mice revealed that 
      21.0-45.9% of the cells were human CD45(+) cells. FISH analysis of frozen 
      sections of transplanted mouse liver revealed the presence of engrafted cells 
      positive for human centromeric DNA. That engrafted human cells functioned as 
      hepatocytes was indicated by the expression of human albumin mRNA, as judged by 
      RT-PCR. FISH analysis with human and mouse centromeric DNA probes excluded 
      spontaneous cell fusion as the cause for the generation of human hepatocytes. 
      Human cord blood cells can give rise to hepatocytes in a xenogeneic 
      transplantation model. This model will be useful to further characterize the cord 
      blood progenitors of hepatocytes.
FAU - Ishikawa, Fumihiko
AU  - Ishikawa F
AD  - Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401, 
      USA.
FAU - Drake, Christopher J
AU  - Drake CJ
FAU - Yang, Su
AU  - Yang S
FAU - Fleming, Paula
AU  - Fleming P
FAU - Minamiguchi, Hitoshi
AU  - Minamiguchi H
FAU - Visconti, Richard P
AU  - Visconti RP
FAU - Crosby, Christopher V
AU  - Crosby CV
FAU - Argraves, W Scott
AU  - Argraves WS
FAU - Harada, Mine
AU  - Harada M
FAU - Key, L Lyndon Jr
AU  - Key LL Jr
FAU - Livingston, Anne G
AU  - Livingston AG
FAU - Wingard, John R
AU  - Wingard JR
FAU - Ogawa, Makio
AU  - Ogawa M
LA  - eng
GR  - P01-CA78582/CA/NCI NIH HHS/United States
GR  - R01-DK54197/DK/NIDDK NIH HHS/United States
GR  - R01-HL52813/HL/NHLBI NIH HHS/United States
GR  - R01-HL57375/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Antigens, CD34)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Bone Marrow Cells
MH  - *Cell Differentiation
MH  - Cell Division
MH  - Fetal Blood/*cytology
MH  - Hepatocytes/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serum Albumin/genetics/metabolism
MH  - Transplantation, Heterologous
EDAT- 2003/06/12 05:00
MHDA- 2003/07/25 05:00
CRDT- 2003/06/12 05:00
PHST- 2003/06/12 05:00 [pubmed]
PHST- 2003/07/25 05:00 [medline]
PHST- 2003/06/12 05:00 [entrez]
AID - 10.1111/j.1749-6632.2003.tb03245.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2003 May;996:174-85. doi: 10.1111/j.1749-6632.2003.tb03245.x.