PMID- 12801578
OWN - NLM
STAT- MEDLINE
DCOM- 20030708
LR  - 20220331
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 79 Suppl 3
DP  - 2003 Jun
TI  - Sperm chromosome aneuploidy analysis in a man with globozoospermia.
PG  - 1662-4
AB  - OBJECTIVE: To determine the frequencies of chromosome aneuploidy and diploidy in 
      sperm from a male with globozoospermia. DESIGN: Assessment of sperm chromosome 
      aneuploidy and diploidy frequencies by multicolor fluorescence in situ 
      hybridization (FISH) analysis. SETTINGS: University research laboratory. 
      PATIENT(S): An infertile patient with round-headed sperm (globozoospermia). 
      INTERVENTION(S): Sperm samples were obtained by masturbation for cytogenetic 
      analysis. MAIN OUTCOME MEASURE(S): Aneuploidy and diploidy frequencies were 
      assessed by multicolor FISH analysis for chromosomes 1, 15, 21, X, and Y and 
      compared with those of five control donors. RESULT(S): A minimum of 10,000 sperm 
      was analyzed per chromosome probe, for a total of 30,145 sperm. There was a 
      statistically significantly increased frequency of XY disomy in the man with 
      globozoospermia compared with the case in normal donors. The frequency of 
      aneuploidy for chromosomes 1, 15, 21, XX, and YY was not statistically 
      significantly increased. The frequency of diploidy was also not statistically 
      significantly different. CONCLUSION(S): A previous report demonstrated an 
      increased frequency of chromosome 15 aneuploidy in sperm of a globozoospermia 
      patient who fathered a trisomy 15 conceptus. No other report has studied the 
      frequency of chromosome 15 aneuploidy in these infertile men. Our study does not 
      demonstrate an increased risk for chromosome 15 aneuploidy associated with 
      globozoospermia. However, an elevated frequency of XY disomy was discovered, 
      which is the most common type of chromosome abnormality observed in sperm of 
      infertile men.
FAU - Martin, Renee H
AU  - Martin RH
AD  - Department of Medical Genetics, Faculty of Medicine, University of Calgary, 
      Calgary, Alberta, Canada. rhmartin@ucalgary.ca
FAU - Greene, Calvin
AU  - Greene C
FAU - Rademaker, Alfred W
AU  - Rademaker AW
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Chromatin)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Chromatin/ultrastructure
MH  - Humans
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Spermatozoa/*abnormalities/*ultrastructure
EDAT- 2003/06/13 05:00
MHDA- 2003/07/09 05:00
CRDT- 2003/06/13 05:00
PHST- 2003/06/13 05:00 [pubmed]
PHST- 2003/07/09 05:00 [medline]
PHST- 2003/06/13 05:00 [entrez]
AID - S0015028203004011 [pii]
AID - 10.1016/s0015-0282(03)00401-1 [doi]
PST - ppublish
SO  - Fertil Steril. 2003 Jun;79 Suppl 3:1662-4. doi: 10.1016/s0015-0282(03)00401-1.