PMID- 12803481 OWN - NLM STAT- MEDLINE DCOM- 20030717 LR - 20240315 IS - 1016-8478 (Print) IS - 1016-8478 (Linking) VI - 15 IP - 2 DP - 2003 Apr 30 TI - The hierarchical relationship among the spitz/Egfr signaling genes in cell fate determination in the Drosophila ventral neuroectoderm. PG - 186-93 AB - The spitz class and Egfr signaling (spi/Egfr) genes are required for the proper establishment of cell fate in the Drosophila ventral neuroectoderm. We investigated the role of the central nervous system (CNS) midline cells, and the hierarchical relationship among the spi/Egfr genes, in this process by analyzing the spatial and temporal expression of several of the genes in selected spi/Egfr mutants. Our analysis showed that expression of all the spi/Egfr genes is severely reduced in the single-minded (sim) mutant, and ectopically induced in en-Gal4/UAS-sim embryos. This result indicates that sim acts upstream of all the other spi/Egfr genes. The CNS midline cells regulate rhomboid (rho) expression in the ventral neuroectoderm and activate the EGFR signaling pathway. We also found that argos (aos) and orthodenticle (otd) act downstream of pointed (pnt), and that aos represses expression of otd in the lateral neuroectoderm to establish differential cell fates in the ventral neuroectoderm. Our findings suggest the following hierarchical relationship among the spi/Egfr genes: [see text]. FAU - Chang, Jinsook AU - Chang J AD - Department of Chemistry, Konkuk University, Seoul 143-701, Korea. FAU - Jeon, Sang-Hak AU - Jeon SH FAU - Kim, Sang Hee AU - Kim SH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cells JT - Molecules and cells JID - 9610936 RN - 0 (Basic Helix-Loop-Helix Transcription Factors) RN - 0 (DNA-Binding Proteins) RN - 0 (Drosophila Proteins) RN - 0 (Homeodomain Proteins) RN - 0 (Membrane Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (Nuclear Proteins) RN - 0 (Proto-Oncogene Proteins) RN - 0 (Rho protein, Drosophila) RN - 0 (Transcription Factors) RN - 0 (oc protein, Drosophila) RN - 0 (pnt protein, Drosophila) RN - 0 (sim protein, Drosophila) RN - 148175-53-5 (spi protein, Drosophila) RN - 62229-50-9 (Epidermal Growth Factor) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Animals MH - Basic Helix-Loop-Helix Transcription Factors MH - Body Patterning/genetics/physiology MH - DNA-Binding Proteins/metabolism MH - Drosophila/*embryology MH - Drosophila Proteins/metabolism MH - Ectoderm/physiology MH - *Epidermal Growth Factor MH - ErbB Receptors/genetics/*metabolism MH - Gene Expression Regulation, Developmental/physiology MH - *Genes, erbB-1 MH - Homeodomain Proteins/metabolism MH - Membrane Proteins/*genetics/metabolism MH - Nerve Tissue Proteins MH - Nuclear Proteins/metabolism MH - Proto-Oncogene Proteins/metabolism MH - Signal Transduction/*physiology MH - Transcription Factors EDAT- 2003/06/14 05:00 MHDA- 2003/07/19 05:00 CRDT- 2003/06/14 05:00 PHST- 2003/06/14 05:00 [pubmed] PHST- 2003/07/19 05:00 [medline] PHST- 2003/06/14 05:00 [entrez] AID - S1016-8478(23)13726-5 [pii] PST - ppublish SO - Mol Cells. 2003 Apr 30;15(2):186-93.