PMID- 12804066
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20030613
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 23
IP  - 5
DP  - 2003 May
TI  - Inflammatory mediators and skeletal muscle injury: a DNA microarray analysis.
PG  - 237-45
AB  - Traumatic skeletal muscle injury causes a specific sequence of cellular events 
      consisting of degeneration, inflammation, regeneration, and fibrosis. The role of 
      early posttraumatic mechanisms, including acute inflammatory response, in muscle 
      repair is not well understood. In the present study, oligonucleotide microarray 
      analyses were used to examine the candidate genes that are involved in these 
      early events of the muscle injury/repair process. cDNA was prepared from the 
      injured and control tibialis anterior (TA) muscle of mice 24 h postinjury and 
      labeled with the fluorescent dye Cy5 or Cy3 prior to hybridization to a DNA 
      microarray. The microarray analysis, including 732 genes, was conducted in 
      triplicate, and we describe only genes modulated by the injury showing a 
      differential expression (both increased and decreased) 1.7-fold or greater (p < 
      0.05) from control uninjured TA muscle. Selected expression patterns were 
      confirmed by other gene expression detection methods, including real-time reverse 
      transcription-polymerase chain reaction (RT-PCR) and RNase protection assay (RPA) 
      or immunohistochemistry detection methods. The upregulated genes (2.8%) were 
      mainly associated with inflammation, oxidative stress, and cell proliferation, 
      whereas the downregulated genes (3.2%) were related to metabolic and cell 
      signaling pathways. In addition, the study suggested that chemokines, such as 
      monocyte chemoattractant protein-1 (MCP-1), associated with monocyte/macrophage 
      influx and activation, are abundantly expressed in postinjured muscle, and they 
      might play a role in traumatic muscle injury/recovery processes.
FAU - Summan, Mukesh
AU  - Summan M
AD  - Toxicology & Molecular Biology Branch, National Institute for Occupational Safety 
      and Health, Morgantown, WV 26505-2888, USA.
FAU - McKinstry, Michael
AU  - McKinstry M
FAU - Warren, Gordon L
AU  - Warren GL
FAU - Hulderman, Tracy
AU  - Hulderman T
FAU - Mishra, Dawn
AU  - Mishra D
FAU - Brumbaugh, Kurt
AU  - Brumbaugh K
FAU - Luster, Michael I
AU  - Luster MI
FAU - Simeonova, Petia P
AU  - Simeonova PP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Chemokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chemokines/genetics
MH  - DNA, Complementary/genetics
MH  - Gene Expression Profiling
MH  - Inflammation Mediators/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*injuries/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/06/14 05:00
MHDA- 2004/01/27 05:00
CRDT- 2003/06/14 05:00
PHST- 2003/06/14 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/06/14 05:00 [entrez]
AID - 10.1089/107999003321829953 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2003 May;23(5):237-45. doi: 
      10.1089/107999003321829953.