PMID- 12805654
OWN - NLM
STAT- MEDLINE
DCOM- 20040802
LR  - 20171116
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 75
IP  - 1
DP  - 2003 Sep
TI  - PCB 104-induced proinflammatory reactions in human vascular endothelial cells: 
      relationship to cancer metastasis and atherogenesis.
PG  - 47-56
AB  - Polychlorinated biphenyls (PCBs) are widespread environmental contaminants that 
      are known to induce carcinogenic and possibly atherogenic events. Recent evidence 
      suggests that selected PCBs may be potent developmental agents of vascular 
      inflammatory responses by inducing cellular oxidative stress and activating 
      redox-responsive transcription factors. Therefore, the aim of this paper is to 
      investigate PCB-induced proinflammatory reactions in human vascular endothelial 
      cells. To determine the proinflammatory effects, cellular oxidative stress and 
      expression of genes encoding for monocyte chemoattractant protein-1 (MCP-1) and 
      adhesion molecules, such as E-selectin and intercellular adhesion molecule-1 
      (ICAM-1), were assessed in human umbilical vein endothelial cells (HUVEC) exposed 
      to 2,2',4,6,6'-pentachlorobiphenyl (PCB 104), a representative of 
      ortho-substituted, non-coplanar PCB congeners. PCB 104 increased the oxidative 
      stress in endothelial cells, as determined by the increased 
      2',7'-dichlorofluorescein (DCF) and rhodamine 123 fluorescence. In addition, PCB 
      104 markedly upregulated the expression of MCP-1, E-selectin, and ICAM-1 at both 
      the mRNA and protein levels. These effects were time- and 
      concentration-dependent. The maximum expression of inflammatory genes was 
      observed in endothelial cells exposed to 20 microM of PCB 104 for 1 or 2 h, 
      depending on the specific gene. In addition, PCB 104 elevated the adhesion of 
      THP-1 cells (a human acute monocytic leukemia cell line) to endothelial cell 
      monolayers. These results indicate that PCB 104 is a potent stimulant of 
      inflammatory mediators in human vascular endothelial cells. We hypothesize that 
      these proinflammatory processes may contribute to the development of cancer 
      metastasis and/or atherogenesis in patients exposed to PCBs.
FAU - Choi, Wangsun
AU  - Choi W
AD  - Department of Surgery, University of Kentucky, Lexington, Kentucky 40536, USA.
FAU - Eum, Sung Yong
AU  - Eum SY
FAU - Lee, Yong Woo
AU  - Lee YW
FAU - Hennig, Bernhard
AU  - Hennig B
FAU - Robertson, Larry W
AU  - Robertson LW
FAU - Toborek, Michal
AU  - Toborek M
LA  - eng
GR  - P42 ES007380/ES/NIEHS NIH HHS/United States
GR  - P42 ES 07380/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030612
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Reactive Oxygen Species)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - Z4YYF101N3 (2,2',4,6,6'-pentachlorobiphenyl)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - E-Selectin/biosynthesis/genetics
MH  - Endothelium, Vascular/cytology/*drug effects/metabolism
MH  - Environmental Pollutants/*toxicity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Inflammation/chemically induced/metabolism
MH  - Intercellular Adhesion Molecule-1/biosynthesis/genetics
MH  - Oxidative Stress
MH  - Polychlorinated Biphenyls/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Up-Regulation
EDAT- 2003/06/14 05:00
MHDA- 2004/08/03 05:00
CRDT- 2003/06/14 05:00
PHST- 2003/06/14 05:00 [pubmed]
PHST- 2004/08/03 05:00 [medline]
PHST- 2003/06/14 05:00 [entrez]
AID - kfg149 [pii]
AID - 10.1093/toxsci/kfg149 [doi]
PST - ppublish
SO  - Toxicol Sci. 2003 Sep;75(1):47-56. doi: 10.1093/toxsci/kfg149. Epub 2003 Jun 12.