PMID- 12806104
OWN - NLM
STAT- MEDLINE
DCOM- 20040318
LR  - 20180610
IS  - 1537-744X (Electronic)
IS  - 2356-6140 (Print)
IS  - 1537-744X (Linking)
VI  - 3
DP  - 2003 May 28
TI  - A calorimetric characterization of Cr(VI)-reducing Arthrobacter oxydans at 
      different phases of the cell growth cycle.
PG  - 432-42
AB  - This is the first of a series of calorimetric studies designed to characterize 
      and understand survival mechanisms of metal-reducing bacteria isolated from 
      metal-polluted environments. In this paper we introduce a new concept of thermal 
      spectrum of the endothermic melting of complex biological systems (e.g., 
      proteins, nucleic acids, ribosomes, membrane structures) in intact cells. All 
      thermal spectra measured are thermograms that describe the temperature dependence 
      of heat capacity change of the complex systems of biologically active substances 
      in bacterial cells. This new concept of thermal spectrum was applied to 
      investigate spectral features from intact cells of Cr(VI)-reducer Arthrobacter 
      oxydans at different points of their growth conditions and stages. Over the 
      temperature range of 40-105 degrees C, we observed that spectral changes are 
      particularly significant in the 40-90 degrees C interval. This may correspond to 
      the orderly changes in subcellular structural elements: proteins, ribosomes and 
      RNA, membranes, and various structural elements of the cell wall during different 
      points of the growth cycle and growth conditions. Spectral changes in the 90-105 
      degrees C region are less pronounced, implicating that the structural composition 
      of DNA-Protein (DNP) complexes may change little.
FAU - Bakradze, Nugzar G
AU  - Bakradze NG
AD  - Department of Physics of Biological Systems, Andronikashvili Institute of 
      Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, Georgia, 380077. 
      bakrn@iph.hepi.edu.ge
FAU - Abuladze, Marina K
AU  - Abuladze MK
FAU - Sokhadze, Victor M
AU  - Sokhadze VM
FAU - Asatiani, Nina V
AU  - Asatiani NV
FAU - Sapojnikova, Nelly A
AU  - Sapojnikova NA
FAU - Kartvelishvili, Tamara M
AU  - Kartvelishvili TM
FAU - Namchevadze, Emma N
AU  - Namchevadze EN
FAU - Tsibakhashvili, Nelly Y
AU  - Tsibakhashvili NY
FAU - Tabatadze, Leila V
AU  - Tabatadze LV
FAU - Lejava, Lia V
AU  - Lejava LV
FAU - Holman, Hoi-Ying
AU  - Holman HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030528
PL  - United States
TA  - ScientificWorldJournal
JT  - TheScientificWorldJournal
JID - 101131163
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0R0008Q3JB (Chromium)
SB  - IM
MH  - Arthrobacter/*chemistry
MH  - Bacterial Proteins/chemistry
MH  - Calorimetry, Differential Scanning/*methods
MH  - *Cell Cycle
MH  - Chromium/*chemistry
MH  - DNA, Bacterial/chemistry
MH  - Oxidation-Reduction
PMC - PMC5974748
EDAT- 2003/06/14 05:00
MHDA- 2004/03/19 05:00
PMCR- 2003/05/27
CRDT- 2003/06/14 05:00
PHST- 2003/06/14 05:00 [pubmed]
PHST- 2004/03/19 05:00 [medline]
PHST- 2003/06/14 05:00 [entrez]
PHST- 2003/05/27 00:00 [pmc-release]
AID - 180657 [pii]
AID - 10.1100/tsw.2003.33 [doi]
PST - epublish
SO  - ScientificWorldJournal. 2003 May 28;3:432-42. doi: 10.1100/tsw.2003.33.