PMID- 12806278
OWN - NLM
STAT- MEDLINE
DCOM- 20040106
LR  - 20191107
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 26
IP  - 3
DP  - 2003 May-Jun
TI  - Large-scale expansion of dendritic cell-primed polyclonal human cytotoxic 
      T-lymphocyte lines using lymphoblastoid cell lines for adoptive immunotherapy.
PG  - 241-56
AB  - Dendritic cells (DCs) have been shown to activate cytotoxic T-lymphocytes (CTLs) 
      for many tumor and virus-associated antigens in vitro. In this study, the authors 
      tested the feasibility of using DCs to expand polyclonal, cytomegalovirus 
      (CMV)-specific CTL lines for adoptive immunotherapy. Two stimulations with DCs 
      expressing pp65, the immunodominant antigen of CMV, effectively activated and 
      expanded MHC-class I restricted, CMV-specific CTLs from peripheral blood 
      mononuclear cells. However, limiting monocyte-derived DC numbers precluded the 
      authors from expanding the CTLs to the numbers required for adoptive transfer 
      protocols. Nonspecific stimulation methods failed to expand CTL lines 
      specifically. However, the authors found that lymphoblastoid cell lines (LCLs) 
      expressing pp65 expanded pp65-specific CTL lines without competition from 
      EBV-specific CTLs. An unlimited source of antigen presenting cells that could 
      present antigen in the appropriate MHC context emerged as a critical point for 
      expansion of polyclonal, antigen-specific CTL lines.
FAU - Sili, Uluhan
AU  - Sili U
AD  - Department of Molecular Virology and Microbiology, Baylor College of Medicine, 
      Houston, Texas 77030, USA.
FAU - Huls, M Helen
AU  - Huls MH
FAU - Davis, Alan R
AU  - Davis AR
FAU - Gottschalk, Stephen
AU  - Gottschalk S
FAU - Brenner, Malcolm K
AU  - Brenner MK
FAU - Heslop, Helen E
AU  - Heslop HE
FAU - Rooney, Cliona M
AU  - Rooney CM
LA  - eng
GR  - R01 CA61834/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Phosphoproteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
SB  - IM
MH  - Cell Line
MH  - Dendritic Cells/*physiology
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods
MH  - *Lymphocyte Activation
MH  - Phosphoproteins/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Matrix Proteins/*immunology
EDAT- 2003/06/14 05:00
MHDA- 2004/01/07 05:00
CRDT- 2003/06/14 05:00
PHST- 2003/06/14 05:00 [pubmed]
PHST- 2004/01/07 05:00 [medline]
PHST- 2003/06/14 05:00 [entrez]
AID - 10.1097/00002371-200305000-00008 [doi]
PST - ppublish
SO  - J Immunother. 2003 May-Jun;26(3):241-56. doi: 10.1097/00002371-200305000-00008.