PMID- 12808017
OWN - NLM
STAT- MEDLINE
DCOM- 20040407
LR  - 20240104
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 116
IP  - Pt 14
DP  - 2003 Jul 15
TI  - Fluorescence in situ hybridization: past, present and future.
PG  - 2833-8
AB  - Fluorescence in situ hybridization (FISH), the assay of choice for localization 
      of specific nucleic acids sequences in native context, is a 20-year-old 
      technology that has developed continuously. Over its maturation, various 
      methodologies and modifications have been introduced to optimize the detection of 
      DNA and RNA. The pervasiveness of this technique is largely because of its wide 
      variety of applications and the relative ease of implementation and performance 
      of in situ studies. Although the basic principles of FISH have remained 
      unchanged, high-sensitivity detection, simultaneous assay of multiple species, 
      and automated data collection and analysis have advanced the field significantly. 
      The introduction of FISH surpassed previously available technology to become a 
      foremost biological assay. Key methodological advances have allowed facile 
      preparation of low-noise hybridization probes, and technological breakthroughs 
      now permit multi-target visualization and quantitative analysis - both factors 
      that have made FISH accessible to all and applicable to any investigation of 
      nucleic acids. In the future, this technique is likely to have significant 
      further impact on live-cell imaging and on medical diagnostics.
FAU - Levsky, Jeffrey M
AU  - Levsky JM
AD  - Department of Anatomy and Structural Biology, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
FAU - Singer, Robert H
AU  - Singer RH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (RNA, Messenger)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - DNA/chemistry
MH  - Genetic Diseases, Inborn/diagnosis/genetics
MH  - *Genetic Techniques
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization/methods
MH  - Publications
MH  - RNA/chemistry
MH  - RNA, Messenger/metabolism
RF  - 61
EDAT- 2003/06/17 05:00
MHDA- 2004/04/08 05:00
CRDT- 2003/06/17 05:00
PHST- 2003/06/17 05:00 [pubmed]
PHST- 2004/04/08 05:00 [medline]
PHST- 2003/06/17 05:00 [entrez]
AID - 116/14/2833 [pii]
AID - 10.1242/jcs.00633 [doi]
PST - ppublish
SO  - J Cell Sci. 2003 Jul 15;116(Pt 14):2833-8. doi: 10.1242/jcs.00633.