PMID- 1280820
OWN - NLM
STAT- MEDLINE
DCOM- 19930107
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 23
DP  - 1992 Dec 1
TI  - Identification of a pathogenic epitope involved in initiation of Heymann 
      nephritis.
PG  - 11179-83
AB  - Heymann nephritis is an experimental autoimmune disease model for human 
      membranous nephropathy. We have recently identified a pathogenic epitope, clone 
      14 (C14), responsible for formation and deposition of glomerular immune complexes 
      that is contained within the small subunit of the Heymann nephritis antigenic 
      complex (HNAC). HNAC is a heterodimer composed of a large subunit designated 
      gp330 and a smaller (44 kDa) subunit, which is immunologically identical to the 
      receptor-associated protein. In this study, we prepared antibodies to fusion 
      proteins with C-terminal deletions in the C14 sequence and assessed their ability 
      to promote formation of immune deposits (IDs). When IgG specific for the shortest 
      truncated fusion protein (C14/delta 3; 86 amino acids) was injected into rats, 
      small IDs developed. In contrast, when IgG raised against the full-length C14 
      sequence was depleted of its reactivity toward the C14/delta 3 fusion protein 
      (C14/delta 3-fp), no IDs could be detected. These data indicate that at least one 
      pathogenic epitope is contained within the N-terminal 86 amino acids of C14. 
      Since the IDs induced with the C14/delta 3-fp-specific IgG are smaller than those 
      induced with the poly-epitope-specific anti-gp330 antibodies, it is likely that 
      other epitopes in addition to those expressed by the C14/delta 3-fp are required 
      for formation and growth of immune complexes.
FAU - Kerjaschki, D
AU  - Kerjaschki D
AD  - Section of Ultrastructural Pathology and Cell Biology, University of Vienna, 
      Austria.
FAU - Ullrich, R
AU  - Ullrich R
FAU - Diem, K
AU  - Diem K
FAU - Pietromonaco, S
AU  - Pietromonaco S
FAU - Orlando, R A
AU  - Orlando RA
FAU - Farquhar, M G
AU  - Farquhar MG
LA  - eng
GR  - DK17724/DK/NIDDK NIH HHS/United States
GR  - NSO7078-13/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (Heymann Nephritis Antigenic Complex)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Autoantibodies/immunology
MH  - Blotting, Western
MH  - DNA Mutational Analysis
MH  - Epitopes
MH  - Glomerulonephritis/*immunology
MH  - Heymann Nephritis Antigenic Complex
MH  - Immunization, Passive
MH  - Kidney Glomerulus/immunology
MH  - Membrane Glycoproteins/chemistry/*immunology
MH  - Rats
MH  - Recombinant Proteins/immunology
MH  - Sequence Deletion
PMC - PMC50513
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
PMCR- 1993/06/01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
AID - 10.1073/pnas.89.23.11179 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11179-83. doi: 
      10.1073/pnas.89.23.11179.