PMID- 1280940
OWN - NLM
STAT- MEDLINE
DCOM- 19930107
LR  - 20190911
IS  - 0148-5016 (Print)
IS  - 0148-5016 (Linking)
VI  - 29
IP  - 2
DP  - 1992 Sep-Oct
TI  - Decondensation of human sperm nuclei and HP1 protamine degradation from 
      normospermia and asthenospermia in Xenopus egg extracts.
PG  - 127-36
AB  - The process of human sperm decondensation has been studied in vitro in 
      cytoplasmic extracts prepared from unfertilized Xenopus laevis eggs. The 
      chromatin decondensation-recondensation cycle was divided into four stages 
      according to chromatin appearance. Spermatozoa from normospermia and 
      asthenospermia were evaluated according to their capacity to reach these stages, 
      and their DNA integrity was assessed by acridine orange (AO) staining. We 
      observed a significant difference between normospermia and asthenozoospermia in 
      the ability to achieve the cycle of chromatin decondensation-recondensation. 
      These results correlated with AO staining. The role of human protamine 1 
      degradation in the decondensation process was evaluated by immunostaining. It was 
      found not to be a prerequisite for the earlier stage of chromatin decondensation 
      and it was not implied in the latest stages of pronuclear development.
FAU - Griveau, J F
AU  - Griveau JF
AD  - Hotel-Dieu, Rennes, France.
FAU - Charbonneau, M
AU  - Charbonneau M
FAU - Blanchard, Y
AU  - Blanchard Y
FAU - Lescoat, D
AU  - Lescoat D
FAU - Le Lannou, D
AU  - Le Lannou D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Arch Androl
JT  - Archives of andrology
JID - 7806755
RN  - 0 (Chromatin)
RN  - 0 (Protamines)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cell Nucleus/*ultrastructure
MH  - Chromatin/*ultrastructure
MH  - Cytoplasm/metabolism
MH  - DNA/analysis
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Infertility, Male/*metabolism/*pathology
MH  - Male
MH  - Ovum/*metabolism
MH  - Protamines/*metabolism
MH  - Spermatozoa/chemistry/*ultrastructure
MH  - Staining and Labeling
MH  - Xenopus laevis
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.3109/01485019208987716 [doi]
PST - ppublish
SO  - Arch Androl. 1992 Sep-Oct;29(2):127-36. doi: 10.3109/01485019208987716.